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Protocols and Workspace
Create a New Protocol
1.
Go to
File
->
New Protocol
2.
A new protocol will open as a new tab in the workspace
3.
From the
Home
tab, you can add a histogram or density plot to your workspace
Opening Protocols
You can open a protocol from either a protocol format file or FCS format file
1.
Go to
File
->
Open Protocol
2.
A Windows dialog will open to locate and open the desired protocol
3.
Search for the protocols directory
4.
To the right of the file name field, there is a dropdown box with the protocol or
FCS file options
5.
If you select the FCS file open, a protocol can also be opened from a saved FCS
file (see below how to perform this in another way)
Saving Protocols
Each protocol is saved with the following information: plots; regions; gates; trigger;
threshold; PMT voltages; sort logic; sort limits; sort modes; compensation settings; filter
configuration
1.
Go to
File
->
Save Protocol
2.
This will save a protocol format file in the currently assigned directory
Loading Instrument Settings
You can load pre-existing instrument settings from a protocol format file into a
currently open protocol
1.
Open the protocol to be used or create a new protocol and save
2.
Go to
File
->
Load Instrument Settings
3.
Select the protocol that contains the settings to be loaded
4.
This will load pre-existing instrument settings into the currently open protocol
