Beckman Coulter Allegra 21 Series Instruction Manual Download Page 67

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Beckman Coulter, Inc.

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250 S. Kraemer Blvd.

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Brea, California 92821

Sales and Service: 1-800-742-2345

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Internet: www.beckmancoulter.com

Summary of Contents for Allegra 21 Series

Page 1: ...IM 8 Allegra 21 Series GS 15 Series and Spinchron 15 Series Centrifuges Instruction Manual IMBALANCE ENTER PULSE ACCEL DECEL TEMP RPM RCF ROTOR TIME STOP START FAST STOP OPEN DOOR SPEED TIME TEMP C AC...

Page 2: ...lter les documents joints Atenci n consulte los documentos adjuntos Attenzione consultare le informazioni allegate On power Ein Netzverbindung Marche mise sous tension Encendido Acceso sotto tensione...

Page 3: ...ich involve the risk of electric shock or personal injury Make sure that the power switch is turned off and the instrument is disconnected from the main power source and refer such servicing to qualif...

Page 4: ...eration may involve the use of solutions and test samples that are pathogenic toxic or radioactive Such materials should not be used in this instrument however unless all necessary safety precautions...

Page 5: ...legra 21 Series GS 15 Series and Spinchron 15 Series Centrifuges Instruction Manual IMBALANCE ENTER PULSE ACCEL DECEL TEMP RPM RCF ROTOR TIME STOP START FAST STOP OPEN DOOR SPEED TIME TEMP C ACC DEC 2...

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Page 7: ...xii Available Rotors xiii SECTION 1 DESCRIPTION Instrument Function and Safety Features 1 1 Instrument Function 1 1 Models 1 2 Safety Features 1 3 Name Rating Plate 1 3 Chassis 1 4 Housing 1 4 Door 1...

Page 8: ...SECTION 4 TROUBLESHOOTING User Messages 4 1 Other Possible Problems 4 3 Emergency Access 4 3 SECTION 5 CARE AND MAINTENANCE Maintenance 5 2 Preventive Maintenance 5 2 Replacing the Rotor Chamber Gask...

Page 9: ...e 2 1 Dimensions of the Nonrefrigerated and Refrigerated Centrifuges 2 3 Figure 3 1 Tapered Sleeve Position 3 3 Figure 3 2 Installing a Rotor 3 4 Figure 3 3 Default Parameters 3 5 Figure 4 1 Emergency...

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Page 11: ...reverse side of the cover page SCOPE OF MANUAL This manual is designed to familiarize you with the Beckman Coulter Allegra 21 series GS 15 series and Spinchron 15 series benchtop centri fuges their f...

Page 12: ...the centrifuge or performing instrument maintenance NOTE If the centrifuge is used in a manner other than spec ified in this manual the safety and performance of this equipment could be impaired Furth...

Page 13: ...tage or risk of electric shock Turn the power switch off and disconnect the equipment from the main power source Refer servicing of all areas displaying either symbol to a service personnel TYPOGRAPHI...

Page 14: ...nterference regu lations of the Canadian Department of Communications Le pr sent appareil num rique n met pas de bruits radio lectriques d passant les limites applicables aux appareils num riques de C...

Page 15: ...5 C Humidity restrictions 95 noncondensing Dimensions Width 42 cm 16 6 in Depth 49 cm 19 3 in Height door closed 38 cm 15 0 in Height door open 80 cm 31 5 in Weight 50 kg 110 3 lb Clearances sides and...

Page 16: ...ation profiles Deceleration 10 deceleration profiles Dimensions Width 42 cm 16 6 in Depth 64 cm 25 2 in Height door closed 38 cm 15 0 in Height door open 80 cm 31 5 in Weight 70 kg 154 4 lb Clearances...

Page 17: ...014 F0850 Fixed Angle 25 Angle 10 000 10 730 9 000 8 690 8 50 346640 GS TB 003 F0685 Fixed Angle 25 Angle 8 000 7 010 8 000 7 010 6 85 364650 GS TB 008 F1010 Fixed Angle 35 Angle 15 300 19 890 14 500...

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Page 19: ...ron 15 series compact benchtop centrifuges generate centrifugal forces required for a wide variety of applications Together with any of several Beckman Coulter rotors designed specifically for use in...

Page 20: ...gerated models also have temperature control systems User messages alert the operator to conditions that may need attention MODELS The centrifuge is available in both nonrefrigerated and refrigerated...

Page 21: ...r monitors the rotor during the run causing auto matic shutdown if rotor loads are severely out of balance At low speeds an incorrectly loaded rotor can cause imbalance Rotor insta bility can also occ...

Page 22: ...nding the drive shaft thermistor and rotor detector are visible in the chamber bottom A gasket system around the chamber opening assures sealing Instrument gaskets have not been designed as bioseals f...

Page 23: ...n the rotor chamber continuously monitors chamber tempera ture The micro processor calculates the required chamber temperature to maintain the selected rotor temperature NOTE In the unlikely event of...

Page 24: ...ays The panel also contains an IMBALANCE light that flashes if rotor loads are severely out of balance Figure 1 4 The Control Panels Power Switch SPEED TIME ACC DEC IMBALANCE ENTER PULSE ACCEL DECEL R...

Page 25: ...T STOP Pressing the FAST STOP key causes the centrifuge to decelerate to a complete stop at the maximum rate The deceleration cannot be inter rupted the centrifuge can only be restarted after the roto...

Page 26: ...arameters ENTER Parameter speed time temperature and acceleration or deceleration curve changes made while a run is in progress must be verified by pressing the ENTER key RPM When the RPM key is press...

Page 27: ...d Time is not counted down and the run will continue until the STOP or FAST STOP key is pressed TEMP refrigerated models only The TEMP key is used to select run temperature on refrigerated model centr...

Page 28: ...te that data can be entered The actual real time operating conditions of the centrifuge are displayed during the run after START is pressed NOTE Error messages see Section 4 also appear on the display...

Page 29: ...e infinity symbol indicating continuous operation lights up and the TIME display shows time elapsed since the run start After 9 hours and 59 minutes the timer will reset to 0 and continue counting ela...

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Page 31: ...nother person INSTALLING THE INSTRUMENT WARNING Do not place the centrifuge near areas containing flammable reagents or combustible fluids Vapors from these materials could enter the centrifuge air sy...

Page 32: ...fuge Allow sufficient drying time before running the centrifuge The centrifuge is shipped with a cone shaped plastic stabilizer installed in the rotor chamber to prevent damage to the drive motor and...

Page 33: ...ensions of the Nonrefrigerated and Refrigerated Centrifuges 42 0 16 6 7 6 3 0 80 0 31 5 38 0 15 0 64 0 25 2 cm 42 0 16 6 7 6 3 0 49 0 19 3 80 0 31 5 38 0 15 0 in Allegra 21R GS 15R or Spinchron 15R Al...

Page 34: ...ith the voltage listed on the name rating plate affixed to the centrifuge Never use a three to two wire plug adapter Never use a two wire extension cord or a two wire non grounding type of multiple ou...

Page 35: ...terials in this centrifuge unless all appropriate safety precautions are taken Always use the appropriate rotors and adapters Handle all infectious samples according to good laboratory practices and m...

Page 36: ...re an experienced user of this centrifuge you can turn to the summary for a quick review of operating steps PREPARATION AND LOADING For fast temperature equilibration cool or warm the rotor to the req...

Page 37: ...t straight down 6 Install the rotor see Figure 3 2 according to the instructions in the appropriate rotor manual Always run the rotor with a balanced load If you are using a horizontal swinging bucket...

Page 38: ...otor straight down onto the drive shaft Tighten the tie down screw clockwise onto the drive shaft Attach the lid and tighten with the T handle wrench Lower the yoke straight down onto the drive shaft...

Page 39: ...s for each rotor that has been centrifuged After the initial use the program parame ters of the latest previous run will be displayed when power is applied NOTE A Program Library is provided at the ba...

Page 40: ...ized rotor number then press the START key an error message will be displayed and the run will be aborted shortly after the rotor starts spinning 1 Press the ROTOR key A rotor number the last rotor pr...

Page 41: ...RPM key The last digit on the SPEED display 0 will flash indicating that the rpm can be entered in 100 rpm increments with the cursor keys 2 Press the or cursor key until the required rpm is displayed...

Page 42: ...down during continuous operation instead the infinity symbol indicating continuous operation lights up and time elapsed since the run start is displayed The run will continue until the STOP or FAST S...

Page 43: ...setting may be needed Acceleration rates are listed in Table 3 1 Table 3 1 Acceleration Deceleration Times in minutes seconds Times are approximate actual times will vary depending on rotor load Maxim...

Page 44: ...can be entered with the cursor keys 2 Press the or cursor key until the required number is displayed STARTING A RUN The run can be started using the parameters in memory from a previous run or using...

Page 45: ...specified time period or from a specified time period to continuous NOTE The deceleration rate cannot be changed after decel eration starts Use the program keys as described under ENTERING RUN PARAME...

Page 46: ...to a complete stop and the door is opened and closed 2 After the rotor stops spinning and the OPEN DOOR light comes on press the OPEN DOOR key to release the door latches then open the door NOTE To p...

Page 47: ...POWER switch to on I Open the centrifuge door press the OPEN DOOR key and lift the door up 2 Make sure that the tapered sleeve is in place at the base of the centri fuge drive shaft before installing...

Page 48: ...time to count down to zero or end the run by pressing either the STOP key or the FAST STOP key 8 After the rotor stops spinning and the OPEN DOOR light comes on press the OPEN DOOR key to release the...

Page 49: ...ervice representatives USER MESSAGES CAUTION If the message SEr appears on the display do not press any keys while the message is displayed Turn the centrifuge power off O and back on I to clear the m...

Page 50: ...then back on I to reset 81 Door open detected during run Maximum deceleration to full stop After rotor comes to a complete stop shut the centrifuge door turn the power off O then back on I to reset 8...

Page 51: ...ng with probable causes and corrective actions required Possible causes for each problem are listed in the probable order of occurrence Perform the recommended corrective action in sequence as listed...

Page 52: ...tor spinning 2 Power not on 3 Source power failure 4 Latch stuck 1 Wait until the rotor stops 2 Plug in the power cord turn power on I 3 See EMERGENCY ACCESS below 4 See EMERGENCY ACCESS below Display...

Page 53: ...e panel see Figure 4 1 4 Rotate the screw clockwise until you hear a click or the door pops open 5 Remove the wrench If the rotor is still spinning close the door and wait until it stops before attemp...

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Page 55: ...y to decontaminate the centrifuge as well as any rotors and accessories before requesting service by Beckman Coulter Field Service representatives WARNING Any maintenance procedure or servicing of thi...

Page 56: ...onge to wipe condensation out of the chamber bowl between runs to prevent chamber icing refrigerated models If chamber icing occurs defrost before use To prevent the rotor from sticking lubricate the...

Page 57: ...thoroughly and dry completely Lubricate the drive shaft with Spinkote after cleaning Wash the bowl using a mild detergent such as Solution 555 Rinse thoroughly and dry completely Clean the centrifuge...

Page 58: ...damage the centrifuge no guarantee of sterility or disinfection is expressed or implied When sterilization or disinfection is a concern consult your laboratory safety officer regarding proper methods...

Page 59: ...UPPLY LIST Refer to the applicable rotor manual for materials and supplies needed for rotors NOTE Publications referenced in this manual can be obtained by calling Beckman Coulter at 1 800 742 2345 in...

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Page 61: ...chart below as a quick reference when duplicating run conditions Refer to Section 3 of this manual for the numbers representing acceleration and deceleration ACCEL DECEL rates Program Number User Dat...

Page 62: ...Program Library A 2 Program Number User Date Purpose Speed Time hrs min Temp C ACCEL Rate DECEL Rate RPM RCF...

Page 63: ...olely by Beckman Coulter REPLACEMENT Any product claimed to be defective must if requested by Beckman Coulter be returned to the factory transportation charges prepaid and will be returned to Buyer wi...

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