Azure c150, c200, c300, c400, c500, c600, User Manual, Page: 13 / 19

Azure™ cSeries User Manual Page 12
For each wavelength, you can set a unique exposure time. Each channel will be captured in a unique
color channel, and will be displayed in a multicolor overlayed image. You can take a 1, 2 or 3 channel
image.
1. Place blot in the center of the Blot Tray.
2. Close the door.
3
A
. Manual Exposure:
• Touch the exposure time box next to the channel that you want to
image. Type in an exposure time using the keypad.
See section 3.2
A
on how to use the keypad.
• To take a multi channel image, such as a Cy5/Cy3 image, enter an
exposure time for both the Cy5 and Cy3 channel.
• For all channels you do not want to image, leave the exposure time at 0.
• Maximum exposure time is 5 minutes per channel.
3
B
. Auto exposure: autoexpose will calculate the best imaging time for you image. After selecting
AUTOEXPOSE , simply press CAPTURE . You can choose auto exposure for all, one, or two
channels.
4. Select the resolution. The higher the resolution, the longer we recommend setting your
exposure time.
5. Select CAPTURE . The channels will be captured sequentially.
6. The resulting image will be displayed in the gallery tab.
3.5 NIR Applications (c500 and c600)
For all NIR blots, use the Blot Tray on the UV transilluminator
position only. The blot tray provides a dark background to
improve the contrast on the blot image. Placing this Tray on
the UV transilluminator position provides even illumination
for your samples.
1. Place blot in the center of the Blot Tray on the UV
transilluminator position.
2. Close the door
3
A
. Manual Exposure:
• Touch the exposure time box next to the channel that you want to
image. Type in an exposure time using the keypad.
See section 3.2
A
on how to use the keypad.
• To take a multi channel image, enter an exposure time for both the 700 and 800 channel.
• For all channels you do not want to image, leave the exposure time at 0.
• Maximum exposure time is 5 minutes per channel.
3
B
. Auto exposure: autoexpose will calculate the best imaging time for you image. After selecting
AUTOEXPOSE , simply press CAPTURE . You can choose auto exposure for all, one, or two
channels.
4. Select the resolution. The higher the resolution, the longer we recommend setting your
exposure time.