Azure™ cSeries User Manual
Page 11
3.3
A
Imaging Workflow for Chemiluminescent Applications
1. Remove the Blot Tray from the cSeries door.
2. After you have applied the substrate to your blot, place your blot in the very center of the tray.
3. Place the blot tray in one of two positions: on the UV transilluminator, or on the chemi shelf, in
the middle of the imaging system.
• Use the position on the UV transilluminator for larger blots.
• Use the position on the chemi shelf for small blots. Using this top shelf increases the
resolution of the blot.
4. Close the door to the system.
5. Select an exposure time. Choose the exposure time manually or use autoexposure which will
allow the software to capture image for you using the full dynamic range of the camera.
• Recommendation: Set the exposure time to 30 Seconds by hitting
3
then
0
and then
S
.
Keep the Sensitivity setting on the default setting,
NORMAL
. Select
CUMULATIVE
. After hitting
CAPTURE
, a series of images will be displayed, and you can select the best one from the
gallery tab.
• Maximum exposure time is 60 minutes.
6. Select
CAPTURE
.
7. The images will be displayed in the gallery tab.
3.4 RGB Applications (c400 and c600)
Selecting
RBG
will give you the follow screen:
The RGB setting is for fluorescent Western blots labeled with
Cy5/Cy3/Cy2 or dyes with similar excitation and emission
spectrums.
For all RBG blots, use the Blot Tray on the UV transilluminator
position only. The blot tray provides a dark background to
improve the contrast on the blot image. Placing this Tray on
the UV transilluminator position provides even illumination
for your samples.
Positions for blot tray:
Chemi Shelf (for chemi blots only)
UV Transilluminator (for all blots)