Installation
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11
Headstage
The AP-1A headstage mates with its connector on the rear panel. To complete
the circuit a ground electrode appropriate for the preparation must be used. A
Ag/AgCl pellet assembly is provided with the Axoporator. You may wish to
make your own ground electrode. The ground electrode may be a chlorided
silver wire dipped into the media bathing a cell culture or an alligator clip
placed on an incision of an
in vivo
preparation. The ground electrode must be
in electrotonic contact with the electroporation micropipette, but the distance
between the two is not important. Connect the ground electrode to the back of
the headstage unit. Use the 2 mm plugs or leads with 2 mm plugs to fashion a
connector to run from the ground electrode to the back of the headstage.
Optional Configurations
Additional inputs and outputs that extend the flexibility of the Axoporator are
available as well.
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To follow the voltage or current signals at the micropipette connect to the
Voutput and Ioutput, respectively.
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To use the audio monitor, plug in a headset or external speaker.
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To trigger a pulse or pulse train with an external TTL device use the
EXT. TRIGGER input.
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To trigger the Axoporator via foot connect the FOOT SWITCH.
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To apply command from an external waveform generator use
EXT COMMAND.
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To synchronize the monitoring device to the 100 Hz resistance pulses and
electroporating pulses use SYNC OUTPUT.
Oscilloscope
An oscilloscope will prove useful should an external waveform generator be used.
See Reference Section Rear Panel Inputs/Outputs for details.
Installation