Reference Section
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23
Figure 8. Inputs/Outputs, bottom row.
V
OUTPUT
:
Electrode voltage (Vo) output. Provides a 0.1V signal for 1V delivered
to micropipette.
EXT COMMAND:
External command option for external waveform generator
.
Although the three pulse types provided with the Axoporator have proven to be
more than sufficient for single-cell electroporation, a signal generator can be used
to deliver virtually any waveform command to the headstage. The signal amplitude
of the waveform must not exceed
±
10 V. When EXT COMMAND is enabled this
will be indicated on the Axoporator display screen.
1. Selecting the external command mode only disables the internal waveform
generator of the Axoporator.
2. An external command always sums with the internal command, even if the
external command mode is set to Off. Thus, no external signal generator
should be connected to the Axoporator, unless it is to be used as the only
source of a command.
The resistance, current and power readings on the display screen will not be valid
when the external command is active. Successful single-cell electroporation is
critically dependent on the micropipette tip voltage and current. So, V
OUTPUT
and
I
OUTPUT
should be used to monitor the command and the current response of the
micropipette. A poor quality current response, drooping waveform, may lead to
sub-optimal electroporation. Also, the resistance at the micropipette tip must be
monitored to ensure that the tip and cleft resistance remain optimal. The resistance
is computed using Ohm’s Law.
I
OUTPUT
:
Current output scaling dependent on headstage: 1V/1 µA or 0.1V/1 µA.
Reference Section
