Introduction
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slices, or
in vivo
. The ability to target individual cells is a great advantage for
experiments designed to test cell-autonomous effects of manipulation. Single-cell
electroporation is especially useful for studies requiring single cell resolution, such
as imaging studies of cell morphology (Haas, 2001; Sin, 2002). The ability of
single-cell electroporation to co-deliver multiple molecules, including multiple
genes to any cell type, makes single-cell electroporation a powerful and versatile
method.
With Axon Instruments’ Axoporator 800A it is possible to electroporate
individually targeted cells. Axoporator 800A provides precise control of the
voltage pulse and train parameters. The result is optimized targeted delivery of ions
or molecules into cells in culture, tissue slice or
in vivo
.
Introduction
