Operation
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5.3
Real-time PCR analysis
5.3.1
Safety instructions
WARNING
Biological hazard!
Take great care when working with potentially infectious material.
Wear suitable protective equipment, e.g. protective gloves.
WARNING
Risk of eye injury!
The rapid heating of the thermal block can cause liquids to evaporate explosively. Al-
ways wear safety goggles during operation.
WARNING
The thermal block, the samples, and the heated lid reach high temperatures. There is a
risk of burns during contact.
5.3.2
Preparing samples
1.
After pipetting the PCR samples into the wells these must be sealed with a transpar-
ent optical adhesive foil (sealing foil).
Note:
The optical transparency of the foils affects the fluorescence signal directly.
Therefore, only use clear adhesive foil as offered for real-time PCR.
2.
Prepare a real-time PCR project with complete details about the PCR run, fluores-
cence measurements and the sample layout of the PCR plate (see qPCRsoft auto
manual).
3.
Move out the drawer and place the sample carrier on it. Make sure that Well A1 is
on the left-hand side.
ATTENTION
Never attempt to pull the drawer out or push it in manually. This may damage the de-
vice.
4.
Start the PCR run. The drawer with the sample carrier is automatically inserted into
the qTOWER³ auto and the PCR program is processed step-by-step.
