Introduction
Agilent Seahorse XFp Glycolytic Rate Assay Kit User Guide
9
Glossary
•
Glycolysis:
In the context of the Seahorse XFp Glycolytic
Rate Assay, the process of converting glucose to lactate.
•
Buffer factor (BF):
Buffer capacity of the measurement
system, comprising the assay medium and XF assay
conditions (instrument, sensor, labware).
•
Proton efflux rate (PER):
The number of protons exported
by cells into the assay medium over time, expressed as
pmol/min.
•
Glycolytic proton efflux rate (glycoPER):
The Proton
Efflux rate derived from glycolysis (discounting the effect of
CO
2
-dependent acidification). This measurement is highly
correlated with the extracellular lactate production rate.
•
Compensatory glycolysis:
The rate of glycolysis in cells
following the addition of mitochondrial inhibitors,
effectively inhibiting oxidative phosphorylation, and driving
compensatory changes in the cell to use glycolysis to meet
the cells' energy demands.
•
Post-2-DG acidification:
This value includes other sources
of extracellular acidification that are not attributed to
glycolysis or mitochondrial TCA activity as well as any
residual glycolysis not fully inhibited by 2-DG. It is
measured after the addition of 2-DG in the Glycolytic Rate
Assay workflow.
•
Induced assay:
Assay workflow that includes an acute
injection of an experimental compound prior to injection of
XF Glycolytic Rate Assay compounds. This workflow allows
for real-time quantification of
in situ
glycolysis activation or
repression.
Summary of Contents for Seahorse XFp
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Page 10: ...10 Agilent Seahorse XFp Glycolytic Rate Assay Kit User Guide Introduction...
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