Agilent Technologies Seahorse XFp, User Manual

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5
Agilent Seahorse XFp Glycolytic Rate Assay Kit
User Guide
Agilent Technologies
1
Introduction
Assay Background 5
Glossary 9
Assay Background
For use with an Agilent Seahorse XFp Extracellular Flux
Analyzer.
The Agilent Seahorse XFp Glycolytic Rate Assay is an accurate
and reliable method for measuring glycolysis in cells. Together
with a Seahorse XFp Analyzer, the Seahorse XFp Glycolytic
Rate Assay provides accurate measurements of glycolytic rates
for basal conditions and compensatory glycolysis following
mitochondrial inhibition (see
Figure 1 on page 6). The
calculated rates accounts for contribution of CO
2
to
extracellular acidification derived from mitochondrial/TCA
cycle activity, and are directly comparable to lactate
accumulation data.