Agilent Technologies Seahorse XFp, User Manual

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18 Agilent Seahorse XFp Glycolytic Rate Assay Kit User Guide
Assay Workflow
Running the Assay
Load template onto the Seahorse XFp Analyzer
If template(s) are already present, skip this step.
1 Visit the Agilent Technologies website, and download the
Seahorse XFp Glycolytic Rate Assay Report Generator (both
Basic and Induced Assay Templates are included in the
downloaded folder).
2
Copy the unzipped Assay Templates (*.asyt) files to the root
directory of a USB Flash Drive.
3 Insert USB drive into the front USB port of the Seahorse
XFp analyzer.
4
Click Settings , then click Template Management .
5
Click the USB tab, and locate the Assay Template(s) to
upload.
6 Click the checkbox next to each Assay Template to be
imported, then click
Import .
7
All imported Assay Templates will now be available for
selection from the ‘Local’ tab when starting an XFp assay.
Run the Seahorse XF Glycolytic Rate Assay
1
Click Start .
2
Select the Seahorse XF Glycolytic Rate Assay or Seahorse XF
Glycolytic Rate Assay (Induced Assay)
template from the Local
tab.
3
Group page: No action required – Confirm or adjust the
groups and plate map, then click the right arrow.
4 Protocol page: No action required – Confirm the protocol
then click the right arrow.
5
Summary page: Change the name of the assay results file, if
desired. Click
Start Assay
when ready.
6 Place the utility plate with the loaded assay cartridge on the
instrument tray and click
Continue . Calibration takes
approximately 20 minutes.
7 When calibration has been completed, remove the utility
plate and place the Seahorse XFp Cell Culture Miniplate on
the tray and click
Continue
to start the assay.