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Microplate Washer User Manual

Chapter 7 Creating a new wash protocol

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18

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Chapter 7 Creating a new wash protocol

Starting from the Protocol File List interface, press the function button F1 to choose “New” to create a
new wash protocol. It is also possible to open an existing protocol from the list, and then modify is
as needed.

Protocol File Interface

Enter a name for the new wash protocol using the number/letter keyboard, then press OK to save it.

The Protocol Programming Interface will be displayed, and using the UP, DOWN, and OK buttons,
the program can be customized.

Summary of Contents for MW9600

Page 1: ...I MW9600 Microplate Washer User Manual Version 1 1 Accuris Instruments is a division of Benchmark Scientific ...

Page 2: ...al check Upon receipt please check the outer package for damage and check all parts inside the package to make sure all parts and components are included and in proper condition If anything is missing or damaged please contact your distributor or Benchmark Scientific immediately Packaging Please keep the box and packaging material for your microplate washer In the event that service is required th...

Page 3: ...ons from Bechmark Scientific s Service Personnel Please contact your distributor or Benchmark Scientific to arrange service or repair This instrument is intended for research only Warning Risk of biological contamination All samples used for testing quality control or calibration should be considered potentially infectious and any parts of the instrument that contact the samples should also be con...

Page 4: ...ect input voltage If the power cable is damaged replace it with same type Turn off the power and unplug the instrument if not being used for a long period of time All bottles should and tubing should be rinsed and emptied When storing cover the instrument to protect it from dust Shut off the power immediately if any of the following occur and then contact your distributor of Benchmark Scientific s...

Page 5: ...Chapter 5 Start Up and Software 8 Chapter 6 Preparing Wash and Waste Bottles 17 Chapter 7 Creating a new wash protocol 18 Chapter 8 Running a wash protocol 22 Chapter 9 Importing Exporting and Deleting Programs 25 Chapter 10 Power Off 28 Chapter 11 Maintenance 29 Chapter 12 Trouble Shooting 30 Appendix MW9600 diagram 31 ...

Page 6: ...ro plates with Flat U V and C type well bottoms Wash head positions are accurately controlled to 0 1mm Multiple wash programs can be set up stored and selected Program allows selecting specific rows for washing Pause function allows pausing the currently running program Wash bottles include clearly marked volume graduations to visually monitor liquid levels Automatic monitoring and warnings when w...

Page 7: ... plates 96 wells standard SBS dimensions Compatible Well bottoms Flat U V C bottom Residual liquid volume 1uL each well Dispensing Volume Range 50uL 2000uL Wash cycles 1 99 Programs 100 Rinse function time range 00 00 03 59 mm ss Soak and shaking time range 00 00 99 00 mm ss Wash bottle level warning Yes sensors included in wash and rinse bottles Wash solution channels 3 bottles Waste bottle level...

Page 8: ...r User Manual Chapter 3 Instrument Overview 3 Chapter 3 Instrument Overview Wash bottle A Wash bottle B Wash bottle R Waste bottle W Transparent Cover Wash LCD display Display housing Rear housing Plate holder Platform ...

Page 9: ...rs Wash head 2 wash heads available 1X8 channel and 1X12 channel 1X12 channel wash head USB port 1X8 channel wash head USB port SD card slot Waste W exit Waste W negative pressure Bottle R inlet Bottle B inlet Bottle A inlet Bottle A fluid level warning Bottle B fluid level warning Bottle R fluid level warning Bottle W fluid level warning Power switch ...

Page 10: ...The packaging is wet Any parts or accessories are missing compare to the packing list Make sure all ordered items are included There is any damage to the Microplate Washer or accessories Installation steps Open the package remove the four bottles wash heads power supply and the instrument and place in onto a sturdy flat and level desk or lab bench Attention Remove the shipping safety lock before o...

Page 11: ...stallation 6 Wash head installation Connect the silicone tubes per the following picture Insert the wash head into the rack and connect the silicone tubing to instrument as pictured below 1X8 and 1X12 wash heads are installed in the same manner ...

Page 12: ...onnection Connect the Washer to the appropriate AC power source Silicone Tube from Instrument Connection Port Color Bottle W non pressure output tube Non pressure Yellow Bottle A fluid output Washing A Black Bottle B fluid output Washing B Green Bottle R fluid Output Rinse R Purple Bottle W fluid input Waste Red Level detection line Instrument Connection Port Bottle A fluid level warning Alert A B...

Page 13: ... insert or remove a flash drive during the self test Do not touch the wash head wash head rack or plate holder when running An alert sound and warning will be displayed if the Waste Bottle W is full or if A B or R are empty or any of the sensors are not properly connected In the event of this type of start up warning check the liquid levels and sensor connections Then push OK or F4 to cancel the w...

Page 14: ...n it will pause the program Browse with the direction keys Push and hold any key for the cursor to move faster in the direction you choose F1 F2 F3 F4 are shortcut keys Push Rinse button in the Wash interface to rinse or prime the tubing Pressing a second time will pause the priming Press F4 key to cancel The stop button is used to stop a program during operation ...

Page 15: ...ile List Use the UP DOWN and OK buttons to highlight an existing saved protocol and press OK to select it Or use the function keys F1 to F4 to select New Delete Import Export or Back The bold numbers and letters on the keypad correspond to the columns of wells on the ELISA plate when selecting the columns to be washed Numbers 1 12 are used for the 1x8 head letters A to H when using the 1x12 head T...

Page 16: ...ll be purged through the tubing during the start of a protocol run Volume can be adjusted from 5ml to 100ml by 5 ml increments The default purge volume is 50ml Note When a protocol is selected that uses a different wash bottle than used in the previously run protocol the purge function will run before the washing steps This purge function will replace the liquid in the dispensing tubes and wash he...

Page 17: ...e instrument is powered on the purge function will run to remove bubbles and prime the dispensing tubes and wash heads This can be set for Single or Multiple Single setting Only the single wash solution that is selected in the protocol will purge through the tubes and wash head prior to washing Multiple setting All of the active wash solutions Solutions A B R when liquid level sensors are enabled ...

Page 18: ... bottle A B or R Set the rinse time 00 01 03 59 mm ss the default time is 2 00 Rinse 1X8 Calibration and 1x12 Calibration It s recommended to calibrate the position of the wash head before use although it has been calibrated in the factory with standard micro plates Vertical and horizontal positioning can be adjusted in increments of 0 1mm Choose 1x8 or 1x12 channel wash head depending on which is...

Page 19: ...ll move so that the wash head needles are aligned above the left row of wells Use the UP or DOWN arrow buttons to move the needles left or right respectively to position the needles directly above the center of the wells Push OK button to confirm and save the setting Right deviation Right deviation means the distance from the starting position of the needles to the center of the right row of wells...

Page 20: ...ush UP and DOWN to adjust the distance The distance between the needle bottom and the top of the micro plate should be approximately 2 3mm Bottom deviation is the distance between the wash head bottom to the photoelectric switch Choose the top deviation function in the calibration menu then push OK button to enter Push UP and DOWN to adjust the distance The distance between the needle bottom to th...

Page 21: ...e languages English and Chinese Reset All settings will be restore factory settings if press Reset except calibration interface Time Date and languages setting System Upgrade MCU Upgrade for hardware updating by USB flash drive Soft Upgrade for software updating by USB falsh drive Picture Upgrade the start up image and home page image can be changed by USB flash drive ...

Page 22: ... bottle and securely connect the tubing to the bottles and appropriate ports on the instrument Use the Rinse function as needed to purge the tubing and remove bubbles before running a program Make sure there is enough solution in the bottles A B R and there is space in the W Waste bottle before running the program Note The system will detect the fluid level automatically Check the fluid level warn...

Page 23: ...on F1 to choose New to create a new wash protocol It is also possible to open an existing protocol from the list and then modify is as needed Protocol File Interface Enter a name for the new wash protocol using the number letter keyboard then press OK to save it The Protocol Programming Interface will be displayed and using the UP DOWN and OK buttons the program can be customized ...

Page 24: ...otocol 19 Well Type can be set per the shape of the well bottoms on the microplate to be used Wash Head Choose the wash head that is installed 1x8 or 1x12 channel Steps Selecting the Steps button will open the Step Programming interface Step Programming Interface ...

Page 25: ...uring shaking Wash Mode Set the wash mode for Full Plate or Column The default is Full Plate o Full Plate In Full Plate mode liquid will be dispensed into all selected columns across the plate there will be a pause for the soak shake then liquid will be aspirated from all selected columns o Column In Column mode selected columns will be washed individually Liquid will be dispensed and then aspirat...

Page 26: ...e columns which need to be washed When using the 1X8 channel wash head use numbers 1 to 8 to select the columns for washing When using 1X12 channel wash head use letters A to H to select the columns for washing Pressing the number or letter for a row a second time will cancel the selection Save Press the F3 button to Save the new program Well type Aspiration Point Settings One Position Two Positio...

Page 27: ...wer On to multiple Select a protocol from the Protocol file list see Chapter 5 Protocol File List or setup a New Protocol see Chapter 7 Creating a new wash protocol Install the 96 wells plate on the plate holder make sure the position and orientation matches the wash head setting When using the 1 8 wash head the A1 position on the plate should be to the left upper corner and when using the 1 12 wa...

Page 28: ...he sensor is turned off in the settings menu the icon is yellow Fluid level detection for Waste Bottle W When the waste bottle becomes full the icon is red When the waste bottle has space the icon is blue A wash protocol run can be stopped at any time by pushing F4 Back button or the run can be paused by pressing the Start Pause button At the end of the wash protocol the micro plate can be removed...

Page 29: ...e button on the panel This will initiate a priming rinse of the selected tubing from bottle A B or R and will run for the selected time It may be necessary to run the Rinse function with all of the solutions used to fully purge the tubing Pressing the Rinse button from the Protocol interface will open the Maintenance Wash interface Press the Rinse button again to initiate the rinse ...

Page 30: ...g and Deleting Programs Import programs from a USB flash drive Insert the USB flash drive From the Program File List interface press the F3 button Impt Expt The below dialog box will be displayed Push the F1 button on the panel to enter the import interface Protocols files stored on the USB drive will be displayed ...

Page 31: ...import and then press F1 to import Export protocol Insert a USB flash drive From the Program File List interface press the F3 button Impt Expt on the panel and a dialog box will open stating USB Drive Found Press the F2 to enter the export interface and the programs stored in internal memory will be displayed ...

Page 32: ...ng Programs 27 Select the protocols to export then press F1 to complete the export to the USB flash drive Delete programs From the Program File List interface highlight a protocol to delete and press the F2 button Delete to delete the file Demo protocols cannot be deleted ...

Page 33: ... the wash heads can become clogged and repair or replacement may be required The included cleaning tool may also be used to clear clogged tubes If the machine will not be used for a long time it is recommended to run the Rinse cycle disconnect the tubing from the bottles and the instrument and run the machine to clear the liquid out of the internal tubing lines Turn off the power using the power s...

Page 34: ...wash head openings using a syringe Or use a soft brush to clean the inside of the openings Filter cleaning The circular filter assembly at the bottom of each dip tube in the reagent bottles can be removed for cleaning Disconnect the silicone tubing from the filter assembly unscrew the top portion of the assembly and all parts can be cleaned using alcohol Plate Holder The base of the plate holder h...

Page 35: ...plate Position settings for wash head not correct calibrate the wash head in the maintenance setting 7 Liquid dispensed into the plate holder Plate is not positioned correctly per wash head selected Adjust the direction of the plate The wash head is not positioned correctly Check the wash head make sure the correct wash head matches the program 8 Liquid dispensing or suction problem Pump is broken...

Page 36: ...oplate Washer User Manual Appendix 31 Appendix MW9600 diagram Main Interface Power switch Drive board Sensors Solenoid valves Main board Y axis motor X axis motor Photoelectric switches Air pump Liquid pump ...

Page 37: ... 32 Note ...

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