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MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide

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please see Section 5.1.

Clicking the “Kinetic Analysis” button, the window on the right side will offer different

calculation options, (see Fig 19).

The calculations available include:

➢

Average/SD/CV%

➢

Integral

➢

Baseline Subtraction

➢

Select Single Reading

➢

Select Reading Range

➢

Maximum Rate

➢

Maximum (Peak)

Fig 19

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Summary of Contents for MR9610

Page 1: ......

Page 2: ...Operation Manual Version 2 0 MR9610 SmartReader UV Vis Microplate Reader This instrument is for research use only ...

Page 3: ...nts as well as all accessories with the packing list when first received and unpackaged If you find any missing please contact your distributor or Accuris Instruments Safety Warning and Guidelines 1 Important information for safe use Please read the manual carefully and operate safely according to all guidelines This instrument is intended for Research Use Only Users should have a clear understand...

Page 4: ...aboratory personnel with the appropriate training Warning Avoid injury Keep your body or any part of body at a safe distance 1 foot or more from the instrument when running The operator should not open or perform any repairs on the Instrument without specific instructions from Accuris Instruments or an authorized agent of Accuris Performing any unauthorized service repairs will result in loss of w...

Page 5: ...f the instrument when you are finished using it Unplug the power cord and cover the instrument with a cloth or plastic sheet to prevent excessing dust from entering the housing Pull the connector plug from the electrical outlet immediately and contact the vendor in the case of Liquid entering the housing Abnormal operation such as any abnormal sound or smell The instrument is dropped or there is a...

Page 6: ...allation 5 1 Initial check 5 2 Installation location and environment 5 3 Installation steps 5 Chapter 5 Operation Guide 7 1 Initial self check 7 2 System settings 8 3 Protocol Management 9 4 Reading a Microplate 12 5 Result processing 18 6 Report exporting 26 7 Power off 27 Chapter 6 Maintenance storage transportation 28 1 Maintenance 28 2 Storage and transportation 28 Chapter 7 Trouble shooting 2...

Page 7: ...displays a representative plate for easy setting of well parameters blank sample standards positive negative quality control and multi value comparisons 5 Dual optical channels one used for sample detection and one used for reference 6 End point method kinetic readings and spectral scanning can be performed 7 Accepts plates with or without lids Top heating prevents condensation 8 Xenon lamp offers...

Page 8: ...h accuracy 2nm Wavelength repeatability 0 2nm Read out range 0 0 4 0 OD Linearity 450nm R2 0 999 0 0 3 0 Accuracy 450nm 1 0 0 003 Abs 0 2 0 2 0 2 0 2 5 Abs Precision 450nm CV 0 5 or SD 0 003 Precision mode CV 1 0 Fast mode Stability 450nm 0 005A 0 0 2 0 2 2 0 2 5 Measurement speed 8 seconds in Fast mode 96 well plate 28 seconds in Precision mode 96 well plate 74 seconds in Precision mode 384 well ...

Page 9: ...nections 1 B type USB port for PC connection 1 Ethernet port 2 A type of USB ports for mouse and printer connections Power requirements Included power adapter DC24V 6 67A 160W AC 100V 240V Dimension W D H 300 500 260mm 11 8 x 19 7 x 10 25 inch Weight kg 15 5kg 34 Lbs ...

Page 10: ...ual Chapter 3 Instrument Structure 4 Chapter 3 Instrument Overview Structure Rear cover Touch screen Housing External Buttons Plate holder Front door Extension module USB Ports LAN Port Power Switch DC Power Inlet B type USB Port Air outlet ...

Page 11: ...dry clean and stable surface allow enough room on the front side so there is space for plate holder drawer to move in and out and also keep 15cm 6 inches of space behind and on the sides for any wires and air flow Environment a The environment for installation should not be excessively humid or dusty b Temperature should be within the range of 10 C 40 C c Relative humidity should be within the ran...

Page 12: ...to connect it according to the above picture The other cord will connect to a standard electrical outlet 100VAC to 230VAC After connecting the power cords switch the power button on the rear of the instrument to the ON position the front panel will cycle through a start up and self test screen Warning Do not connect the instrument to a power outlet that is not properly grounded ...

Page 13: ...This chapter explains a standard protocol set up and operation beginning with the automatic self check after power on The following screen image will appear during the self check Fig 2 The user login interface will appear after the self check see Fig 3 This allows login as the Admin existing user or click Guest Fig 3 ...

Page 14: ...iles under the user account Guest Cannot be deleted No password Note Please keep the Admin password recorded and in a safe place and contact Accuris Instruments or your distributor if the Admin password is lost or forgotten After logging in the Home Interface screen is displayed Fig 4 Fig 4 The Admin button on the lower left is for the logout and login interface 2 System settings In the Systems se...

Page 15: ...ome button on the top left corner to go to the main interface 3 Protocol Management Click Absorbance on the home interface to enter the layout interface fig 6 This interface is composed of six sections the navigation bar at the top the sidebar the main display area the optional bar setting type selection and the status bar at the lower right area The SmartReader UV Vis incorporates a touch screen ...

Page 16: ...tocol has not been saved Save the current protocol to the protocol list Shortcut for exporting raw data to a flash drive in csv format Exports only the data displayed in the Main Display Area Open the Protocol folder for accessing saved protocols sorting deleting importing exporting and renaming protocols The Protocol list opens on the right side of the screen and can be closed by clicking on the ...

Page 17: ...sh drive to the instrument Rename Change the name of a protocol Save as Save as a new protocol Edit Edit button is located on the top right corner see Fig 7 to enter the edit state Account Protocols of other accounts can be checked but this function is only available for the Admin account When the protocol list in the edit state the below operations are available Sorting protocols can be sorted ac...

Page 18: ... the plate layout to choose the required layout format Fig 8 The plate layout menu allows choosing 6 well setting options as well as a clear option Choose the appropriate setting type and the option bar will change accordingly then click the corresponding wells on the main display area to finish the settings Note the entire plate all wells can be selected by clicking the blank box at the upper lef...

Page 19: ...ng the corresponding wells Example If the set concentration is 125ng μL the operator is multiply and the step size is 2 the concentration of the first well will be 125ng μL the second well 250ng μL 2 x 125ng μl and the third 500ng μL 2 x 250ng μL etc Quality control Settings are done in the same manner as the wells of standard samples Unknown samples Users can set wells as unknown and there in an ...

Page 20: ...set up the layout of the plate The absorbance value data and layout are separate users can create or modify a layout to analyze absorbance data that was captured in the past Note the absorbance values of a test cannot be changed 4 2 Parameter setting After completing the plate layout settings click Run on the left side bar see Fig 10 Fig 10 After clicking Run the lower window becomes a controller ...

Page 21: ...ate in out control the motorized plate drawer to move it in and out there is also a physical control button on the front of the instrument Start Stop Used to start or stop a plate reading There is also a physical button on the front of the enclosure for start stop Shake This setting is independent from the shaking function in the settings menu it is specifically used for plate defoaming In Run mod...

Page 22: ...d Time Shake Speed Low Medium High Type Continuous Pulsed Time Wait Time at start Wait Time at start Wait Time at start Area Selection Area Selection Area Selection For wavelength selection Endpoint and Kinetic are configured with four wavelengths defaults are 405nm 450nm 492nm and 630nm users can click on and modify these wavelength values manually but must be within the range of 200nm 1000nm Spe...

Page 23: ...cation See Fig 11 Fig 11 Click button on the display or press the Start button on the front of the instrument If the protocol has already been run a window will pop up to allow re naming the protocol results file Input a file name click ok to run the protocol The plate drawer will close and during the reading process the screen will turn grey see Fig 12 and all buttons will become inoperable with ...

Page 24: ...bance values measured To view the analyzed results switch to the Result interface by pressing the Result button on the side bar see Fig 13 Fig 13 Results are displayed according to the protocol settings and measurement method The above Fig 13 shows results of an endpoint reading Here according to different types of detection modes the Result is divided into three modes Endpoint Kinetic and Spectru...

Page 25: ...bsorbance values for each sample well Note 1 The Blank Subtraction button is only available if blanks have been set in the protocol layout interface 2 When a protocol layout is set with blank samples the absorbance values of Basic Calculation Standard curve Classification Quality Control and Kinetic Analysis all include the subtraction of the blank value Basic Calculation the four basic functions ...

Page 26: ... to 1 0 this indicates an optimized curve fit Linear Linear Linear fitting of absorbance value and corresponding concentration Linear Log Linear fitting of absorbance value and the logarithm of the concentration Log Linear Log Log Note In order to generate a curve standard wells must be set up on the Protocol Layout Interface Some curve types require a minimum quantity of standards In the event of...

Page 27: ...t be available See Fig 15 Quality Control Click Quality Control button see Fig 16 the main display area will turn out to be a list it displays status of the quality control wells which have set previously the instrument will mark the samples according to conditions set in Parameters Parameters Fig 16 Note protocol layout interface must be set with Quality wells otherwise this button will be not av...

Page 28: ...e the detailed absorbance results for a specific well select the target well then click the Graph button at the top right to enlarge the kinetic curve see Fig 18 Click Back to return to the main interface Fig 18 The Calculation window at the bottom offers four selections Raw Data Blank Subtraction Basic Calculation and Kinetic Analysis see Fig 18 For functions and algorithms of Raw Data Blank Subt...

Page 29: ...Section 5 1 Clicking the Kinetic Analysis button the window on the right side will offer different calculation options see Fig 19 The calculations available include Average SD CV Integral Baseline Subtraction Select Single Reading Select Reading Range Maximum Rate Maximum Peak Fig 19 ...

Page 30: ...ce Click Spectral Analysis see Fig 21 for further analysis Calculations available Spectral Maximum read the maximum value greater than the threshold value within a set range Spectral Normalization set the spectral range take the maximum absorption peak as 1 the remaining values will be converted into percentage based on this criterion Ratio within the Spectrum set two wavelength values λ1 and λ2 c...

Page 31: ...e measurement value of a single wavelength Fig 21a Fig 21b Description of each button on the upper right corner of the main display area Print the current content of the main display area to a connected thermal printer Export the current content of the main display area as an image file to a flash drive ...

Page 32: ...yout view to a data list display click the Plate button in the upper right corner to switch back to the plate layout display Restore the zoom function Return to the previous interface 6 Report exporting Both processed data and raw data can be exported click the Report button on the left side menu see Fig 22 Fig 22 Choose the desired format in the File Type window at the bottom four formats are ava...

Page 33: ...to export data to a flash drive Print The print function can only be used to print the instrument s basic information including instrument serial number software version etc 7 Power off Press the Plate in out button to remove the ELISA plate from the Reader then press the button again to insert the empty plate holder back into the chamber Turn off the power switch on the back of the instrument ...

Page 34: ...ly as this can damage the electronics Make sure to connect the SmartReader to an appropriate power outlet voltage and Hz as stated on the instrument rating plate Maintenance list Content Daily Weekly Annually When needed Make sure the instrument powers off correctly Keep the instrument away from dust Remove spilled solutions right away to prevent damage then clean it with distilled water If any su...

Page 35: ...your distributor or Accuris Instruments 6 E612 E622 E632 E642 when self checking Detection module failure Please contact your distributor or Accuris Instruments 7 E401 E403 E415 E425 E435 E445 when self checking Motor failure Please contact your distributor or Accuris Instruments 8 E011 E056 when self checking Incubation failure Please contact your distributor or Accuris Instruments 9 Test results...

Page 36: ...e Check that the plate is properly seated if liquid has spilled out and whether the front door is working correctly then re start the instrument If problem still occurs contact your distributor or Accuris Instruments 13 Instrument stops running during detection Communication breakpoint Press stop restart the detection ...

Page 37: ...ies 31 Chapter 8 Accessories No Item Type Unit Qty Remarks 1 Power cord PCS 1 2 AC DC Power Adapter 24V 160W PCS 1 3 Mouse Logitech PCS 1 4 Performance test statement PCS 1 5 Operation Manual PCS 1 6 Packing List PCS 1 7 Flash Drive PCS 1 8 Certification PCS 1 9 ...

Page 38: ...32 Memo ...

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