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3M © 2003  78-8078-8977-5                           Page 6 of 9 

Attest 290 Auto-reader 

 

INSTRUCTIONS FOR USE 

 
Positive Control 

 
Crush, tap and incubate at least one non-processed  
Attest

 

1291 or 1292 RRBI to use as the positive 

control each day that a processed RRBI is incubated 
and read by the unit. Write a “C” (for “control”) and 
the date on the label. The positive control can be 
placed in any incubator well of the unit. The 
positive control should be the same part number, 
from the same manufacturing date and lot number 
as the processed RRBI in the unit. 

It is good practice to use a positive control each day 
a sterilized RRBI is processed. This helps ensure: 

correct incubation temperatures are met

.

 

viability of spores has not been altered due to 
improper storage temperature, humidity, or 
proximity to chemicals 

capability of media to promote rapid growth, 
and 

proper functioning of Attest 290 Auto-reader 
components. 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 
Interpretation of Results 

 
Each incubation/reader well has three corresponding 
indicator lights: a red (+), yellow (•) and green (-) 
light. After placing a RRBI into a corresponding 
color-coded well, a yellow light will illuminate to 
indicate that incubation/reading is in progress.  
 
A red light (+), will illuminate and an alarm will 
sound (if the sound feature is turned on), as soon as 
a positive RRBI result is detected. 
 
At the end of the specified incubation time, if a 
negative RRBI result is detected, the green light (-) 
will illuminate indicating an acceptable sterilization 
process. 
 
After either the green or red light comes on, the 
RRBI can be removed from the well. The RRBI 
should not be removed or touched when the yellow 
light is on. 
 
The positive (unprocessed) control RRBI must 
provide a positive fluorescent result (red light +). 
Processed RRBI results are not valid until the 
positive control reads fluorescent positive (red light 
+). With the processed RRBI, a positive (red light 
+) result means a sterilization process failure may 
have occurred. 

The final negative processed RRBI reading for a 
fluorescence change (green light -) after 1 hour 
(1291) or 3 hours (1292) indicates an acceptable 
sterilization process. The positive control should 
read positive (red light +). 
 
If the processed RRBI is further incubated for a 
visual pH color change, when special studies are 
desired, the media color change from purple to 
yellow will also indicate a sterilization failure. The 
positive control should show a yellow color change 
at 24 hours (1291) or 48 hours (1292). A final 
negative color change result (no color change), 
media remains purple , is made at 24 hours (1291) 
or 48 hours (1292). 
 
Act on any positive result immediately. The positive 
result indicates a sterilization process failure may 
have occurred.  Determine the cause of the positive 
RRBI following current facility policies and 
procedures.  Always retest the sterilizer and do not 
use the sterilizer for processing loads until 3 
consecutive RRBI results are negative. 
 
 
 
 

 

 

 

 

 

 

 

 

 

 

 

 

 

 
Disposal 

 
Dispose of the used Attest RRBI according to your 
healthcare facility policy. 

You may wish to sterilize any positive RRBI prior 
to disposal. 

 

 
 

Summary of Contents for Attest 290

Page 1: ...3M Attest 290 Auto reader Operator s Manual Read and understand all safety information before using this product ...

Page 2: ......

Page 3: ...tTM 1292 Rapid Readout Biological Indicators at 60o C for a final negative fluorescence reading at 1 hour for 1291 or 3 hours for 1292 The 3MTM AttestTM 290 Auto reader is also designed to allow for further incubation of the 3MTM AttestTM 1291 RRBI and or 3MTM AttestTM 1292 RRBI for a final negative visual pH color change of the growth media at 24 hours for 1291 and 48 hours for 1292 A positive fl...

Page 4: ...inside the plastic vial of the RRBI To avoid the risk of injury from flying debris due to a ruptured RRBI which if not avoided could result in serious injury or death Allow the RRBI to cool for the recommended time period before crushing Crushing or excessive handling of the biological indicator before cooling may cause the glass ampule to burst Wear safety glasses and gloves when removing the RRB...

Page 5: ...he Attest 290 Auto reader complies with IEC EN 61326 1 as confirmed in the Certificate of Compliance generated by 3M The Attest 290 Auto reader complies with the EMC requirements of the CE mark EMC Directive 89 336 EEC The Attest 290 Auto reader complies with the Australian EMC requirements as confirmed in the Supplier s Declaration of Conformity that is linked to the C tick mark Note This equipme...

Page 6: ...w this procedure for every RRBI to be read Step 1 While wearing gloves and safety glasses CLOSE RRBI cap by pressing down Step 2 CRUSH the glass ampule containing growth media in the designated crushing well built into the incubator Step 3 Hold the RRBI by the cap and TAP bottom of the vial on a tabletop until media wets spore strip at bottom of vial Do not tap vial on unit Step 4 Open the cover a...

Page 7: ...ation period A positive result will automatically be shown as soon as it is detected The full incubation period is required before a negative result is shown The Attest 1291 RRBI requires 1 hour of incubation and the Attest 1292 RRBI requires 3 hours of incubation before a negative result will be shown The negative result will be shown automatically after completion of the required incubation time...

Page 8: ...dicating an acceptable sterilization process After either the green or red light comes on the RRBI can be removed from the well The RRBI should not be removed or touched when the yellow light is on The positive unprocessed control RRBI must provide a positive fluorescent result red light Processed RRBI results are not valid until the positive control reads fluorescent positive red light With the p...

Page 9: ...l will turn off indicating that the alarm is again active Time Remaining When the time remaining button is pressed the incubation time remaining for each individual incubation reader well that contains a RRBI will be displayed All three lights for each individual incubation reader well will illuminate as the time remaining is displayed The process begins with the incubation reader well containing ...

Page 10: ...d before incubation Replace RRBI in proper well within 10 seconds complete All three lights for affected RRBI will light and alarm will sound if active E1 Temperature Control Error Call for repair or replacement This error will occur if system is no longer able to control the temperature of the incubator block 60ºC 2ºC E3 Memory Error Call for repair or replacement E4 Analog Digital Conversion Err...

Page 11: ...Other Negative Control Vial Vial not crushed Crush vial before inserting into well Media did not wet the spore strip Tap vial on table until media wets spore strip Control vial was sterilized Use a non sterilized vial as a control Check chemical indicator on label Other Unexplained positive Adhesive residue on vial Do not place tape or labels on vial Test vial is absorbing fluorescent Place the vi...

Page 12: ...ing 275 4E 01 St Paul MN 55144 1000 1 800 228 3957 3M Health Care D 41460 Neuss Germany Recycled paper 40 pre consumer 10 post consumer Litho in U S A with 3M film proofing systems and offset plates Attest is a registered trademark of 3M 3M 2003 78 8078 8977 5 Rev D Lit Code 70 2009 4089 1 ...

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