Imaging and Flow Cytometry Core CPOS
LSM 800 SOP
C-4
ver. 2.1 (Updated: 11/13/2020)
C.
System start up
1.
Toggle main power supply on wall (labelled
○
1
…..
wait 5 seconds
….
and
○
2
)
2.
Turn on PSU #
○
3
and
○
4
.
3.
Turn on
Laser module
“LM”
turn-key
○
5
.
4.
Turn on power for Metal halide lamp for finding
your specimen under
fluorescent light
○
6
.
5.
Turn ON computer
○
7
.
6.
Double click on the program icon on desktop.
Or
click on Start | All Programs | Carl Zeiss
Microscopy | ZEN | ZEN (blue edition) entry
(blue icon).
7.
Click on “ZEN
system
” button.
8.
Check that you have:
*took out sample frame*
**
adjusted objective lens to 10X or lower
**
before you proceed with “Calibrate Now”
button.
9.
(Optional)
Live cell incubator procedure as follows:
i.
Turn ON Tokai HIT controller module
○
8
.
ii.
Turn gas tank valve counter-clockwise
○
9
.
Read second regulator gauge to confirm gas
supply pressure is at ~17 psi (blue line). Do not
adjust the knob if it is at desired pressure.
8
9
