Imaging and Flow Cytometry Core CPOS
LSM 800 SOP
K-18
ver. 2.1 (Updated: 11/13/2020)
12.
Reference to Chapter [E] 'locate specimen,
focus, find cells/region of interest'
13.
Go to 'Locate' tab.
14.
Turn on fluorescence channel for navigation.
15.
Use xy-motorized control to navigate your
specimen.
16.
When you have a region of interest in view, go
to 'Acquisition' tab.
17.
Turn ON 'Live' view set Focus until image is clear.
Critical
…K16
…K17
18.
GoTo 'Tile' window positions section and click on
'+' under positions.
19.
Repeat steps [K12] to step[K17] until you have
all of the positions required.
20.
Press ‘Start Experiment’
K16
