Imaging and Flow Cytometry Core CPOS
LSM 800 SOP
L-21
ver. 2.1 (Updated: 11/13/2020)
20.
If no Support points were selected, then Tile
will be acquired according to current z-
height. Support points are useful when the
specimen plane is uneven / tilted.
One should always ensure their
specimen is level before attempting to
rescue with “support points” function.
21.
[Optional Guide] Go to 'Support Points' tab.
22.
Set number of columns and rows of support
points. General guide: usually one support
point is enough per three tiles. Suggestion =
~1 support point for 3 tiles in one dimension.
E.g. 6 x 12 tiles required then add minimum
of 2 x 4 support points.
23.
click 'Distribute'
24.
If some of the support points are not in the
area of your specimen you may drag the
yellow marks [insert icon] so that they are
above your specimen.
25.
Verify Tile positions (the height of each
support points) !! skip forward to Step [#].
26.
Click 'Start Experiment'
Advanced Positions
27.
Go to 'Position Setup' tab under advanced
tile preview area.
28.
Navigate on your preview scan image by
double clicks on the image. Motorized XY
drive will bring your specimen in view.
29.
30.
31.
(Optional): bring your specimen into sharp
focus with focus knob. (another method will
be covered in later section)
32.
Use plus sign tool
, select on
navigation area to mark positions you wish
L21
L22
L23
From
To
L27
