Imaging and Flow Cytometry Core CPOS
LSM 800 SOP
J-16
ver. 2.1 (Updated: 11/13/2020)
19.
Adjust laser power so that max image intensity
reach ~
5000 - 8000 intensity units (around 50%
of detector dynamic range) under 16-bit mode or
~30 intensity units under 8-bit mode.
20.
**Important information. For proper
downstream Airyscan processing and preventing
damage to detector. Avoid over-saturating the
Airyscan detector. (intensity must be <8000
units). **
21.
‘
Stop
’
Continuous Scan.
22.
Select a new channel unselect previous channel.
Repeat step [J12] to step [J19] until all channel
has been optimized.
23.
Check ALL channels.
24.
Turn on 'Continuous' scan mode.
…J24
25.
Go to 'Airyscan' tab in centre preview area.
26.
Check 'Detector' under Airyscan tab. Wait for
the detector to be aligned.
*Advanced: you may do this step during intensity
setup.*
If cannot perform this step seek help from
technical staff.
[Refer to step J15]
example for aligned detector
27.
Please check if software shows any warning
about Airyscan acquisition.
28.
Slower scan speed if required
29.
Change back to unidirectional scanning if
required.
30.
Click 'New' and 'Snap' for a single optical section.
Or 'Start Experiment if you have any other
modules activated.
31.
Refer to 'Saving an Image File' (Insert Chapter
number).
J26
J30
