Zeiss LSM 510, Быстрый старт

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10. Advanced Options
10.1. Single-Track – simultaneous acquisition
Multi-track
can solve the problem of cross-talk. Typically this occurs with bleed
through of green fluorescence in to the red channel. Multi Track avoids this by acquiring
the fluorescent channels sequentially, not simultaneously as with
Single Track
acquisition. In
Multi Track
mode the red channel detector is turned on, the green one
off. Then the red fluorophore is imaged.
The red channel detector is then turned off, the green one on and the green fluorophore
imaged. Any bleed through of fluorescence from the green fluorophore to the red detector
does not register.
This switching between tracks means that there is a delay between channels. Sometimes
this is unacceptable, in particular for live cell imaging where the cell can move between
channels creating artefacts. In this case
Single track
mode should be used.
•
To load pre-designated configurations
click the Config button in the
Configuration control window.
10.1.1. Getting the display ready
For multi-label experiments, it is best to have both channels
displayed at once.
To do this:
In the Live image window’s
Display
side-bar, click the
Split
xy
button
This will make your image small so resize the image window
(usual Windows mouse-drag). Then click on the Zoom button to
bring up the Zoom sidebar and select
Auto button.
10.1.2. Optimise settings
Unlike Sequential acquisition (Multi-Track), each channel is acquired simultaneously
during optimization – so be quick!
Refer to section 7.2 for instructions on how to optimize the channel settings.