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19
In the
Scan
control window, click the
Z-
slice
button to bring up the
Optical Slice
dialog
Click the
Optimal Interval
button. Check the
optical section
for each channel is the same and close. If you optical
sections are different for each wavelength go to
Mode/Channels
and adjust the
Pinhole
so that each
channel has the same optical section – around 1 Airy
unit, but one channel will have to be slightly larger.
Readjust the red lines that indicate the top and bottom of the specimen (Step 7 above).
Reduce the
Frame Size
also if possible to speed stack-acquisition.
To acquire your z-series stack click the
Start
button.
The system will begin
scanning the specimen.
You can check progress by
selecting
Gallery
button
on the image window
sidebar.
Содержание LSM 510
Страница 1: ...Quick Start Zeiss LSM 510...
Страница 11: ...11 Your image window will now look like this...