Zeiss LSM 510, Быстрый старт

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7.2. Scan control: Channels window
Click on the
Channels
button of the
Scan control window.
The active channel will appear here.
The settings below need to be adjusted
for each channel separately.
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1. Set Pinhole of each channel:
The pinhole diameter determines the thickness of the optical section – i.e. the axial
resolution
. By increasing the pinhole diameter (and therefore optical section) you are
also increasing the amount of out of focus fluorescence being detected. Start with
each at the optimum (1 Airy Unit) by clicking the
1 button .
This will give different Optical Slice values for each channel. Increase the pinholes
of the shorter wavelength channels to match the longest wavelength.
Do not go below 1 Airy unit – you will lose signal with no significant improvement
in axial resolution. Adjust the Pinhole for each channel so that they have the same
optical slice (typically the green channel will be ~ 1.1 Airy units).
2.
Check focal plane
Click the
Fast scan
button to start acquiring a high-speed, low quality image. Use this mode to focus up
and down through the specimen to get the desired focal plane.
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