Zeiss LSM 510, Быстрый старт

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18
9. Acquiring a Z-series
Having set the system to acquire a satisfactory image, you can acquire a z-series. It may
be worthwhile changing the frame size to 512×512 to minimize file size and to speed
acquisition.
Click on the
Z-Stack
button in the
Scan control
window.
Using
Fast scan
mode to acquire a continuous image of the
specimen. Focus up and down with the
microscope focus wheel to ensure your
specimen is in the centre of the field of view
and the microscope is focused in the middle of
the specimen.
Stop acquisition.
You can now precisely define the middle and
depth of you z-series.
Ensure the
Num Slices
= 20 and the
Interval
= 1 µm at this point to ensure the top
and bottom of the specimen is located.
Click the
Range
button from the Scan control window – this
will generate a side view of you specimen.
This button is not available if the
MarkFirst/Last
button is depressed.
(LEFT) The green line represents the
middle of your z-series. The upper red
line represents the top and the lower red
line the bottom of the z-series.
(RIGHT) Drag the green line to the
centre of the specimen and the red lines
to just outside the top and bottom of the
specimen.