INTRODUCTION TO LASER SCANNING MICROSCOPY
LSM 510
Carl Zeiss
Performance Features of the LSM 510
LSM 510 META
3-8
B 45-0008 e 10/02
It is possible to rotate the XY scanning field through 360° and carry out XY scans without having to
rotate the specimen itself under laser radiation load.
Selection of the specimen detail of interest for zooming is fast and convenient, and the zoomed image is
automatically centered. This saves the job of specimen centration with the microscope stage.
Using a bi-directional scanning facility will double the scanning rate to approx. 5 frames/sec (at 512
×
512 pixels); if two different laser wavelengths are used for the two scanning directions (wavelength 1 for
left-to-right, and wavelength 2 for right-to-left scanning), two fluorochrome dyes can be viewed and
documented in a quasi-simultaneous mode. This will absolutely prevent "bleeding".
3.4.2
Microscope Equipment of the LSM 510 System
The LSM 510 system is equipped either with the inverted Axiovert 200 M BP or SP microscope or with
the upright Axioplan 2 imaging
MOT
, Axiotron 2 or Axioskop 2 FS mot microscopes.
Only the differences from the delivered operating manual "Axiovert 200 M" will be explained here.
(1) Stand
a)
The motorized objective nosepiece 5
×
H DIC is firmly fixed to the stand, where no operating elements
can be found for the nosepiece. Operation will be performed via LSM 5 software control. The
"Restriction of the nosepiece height to protect the objectives during motorized objective change" is
inactivated. The nosepiece will be moved down automatically before each motorized objective change.
b)
The reflector mount is motorized and provided with the Axiovert 200 reflector turret. The reflector
turret has 5 positions: One transmitting light position, which is identical to the LSM position, and four
further positions for fluorescence filter sets (reflector modules). If you want to use more than five
conventional fluorescence filter sets, it is advisable to use a further reflector turret. When changing the
reflector turret position you must make sure that the turret will click into position, since otherwise the
image area will be cut.
c)
The stand has a motorized focusing drive (fine coarse). Sensitivity of the focusing drive is adjusted to
the delivered objectives by the manufacturer. If you want to use other objectives, sensitivity and
parfocality can be adjusted via the Axioset program.
d)
The stand features an integrated power supply for the internal motors and stand electronics. The
power supply can be switched on at the right side of the stand. External power supply units will be used
for the mercury vapor short arc lamp.
Содержание LSM 510 META
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Страница 10: ...NOTES ON DEVICE SAFETY LSM 510 Carl Zeiss Contents LSM 510 META 1 2 B 45 0008 e 10 02...
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Страница 22: ...LSM 510 SETUP REQUIREMENTS LSM 510 Carl Zeiss Contents LSM 510 META 2 2 B 45 0008 e 10 02...
Страница 38: ...LSM 510 SETUP REQUIREMENTS LSM 510 Carl Zeiss System Overview LSM 510 META LSM 510 META 2 18 B 45 0008 e 10 02...
Страница 42: ...INTRODUCTION TO LASER SCANNING MICROSCOPY LSM 510 Carl Zeiss Contents LSM 510 META 3 2 B 45 0008 e 10 02...
Страница 52: ...QUICKSTART LSM 510 Carl Zeiss Contents LSM 510 META 4 2 B 45 0008 e 10 02...