Zeiss LSM 510 META, Руководство по эксплуатации

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LSM 510
INTRODUCTION TO LASER SCANNING MICROSCOPY
LSM 510 META Performance Features of the LSM 510 Carl Zeiss
B 45-0008 e 10/02
3-7
3.4 Performance Features of the LSM 510
3.4.1 Optical and Mechanical Aspects
The highly integrated system design makes for the shortest possible optical paths, top-grade optical
precision and high stability. The compact scanning module can be fitted to an inverted (Axiovert 200 M
BP or SP) or upright (Axioplan 2 imaging
MOT
) microscope in less than three minutes. On the Axiovert,
the scanning module may be mounted either to the base port directly below the microscope or to the
side port.
The spectral range available extends from the UV to the IR region.
For the VIS (visible-light) Laser Module, the user can select from up to five lasers with wavelengths of
633, 568, 543, 514, 488, 477, 458 and 405 nm. The UV Laser Module provides wavelengths of 351 and
364 nm. Coupling of the laser light is through polarization-preserving single-mode optical fibers. One
variable beam collimator each for the UV and visible ranges provides optimum adaptation of the
respective laser wavelength to the objective used and, thus, optimum correction for Z aberrations.
Acousto-optical tunable filters (AOTF) adjust the necessary brightness for up to 6 desired laser lines
within microseconds.
A monitor diode permanently registers the laser output; it can be used for the on-line checking of the
intensity of the exciting light. This check is also possible selectively for the different wavelengths if a line
selection filter is inserted.
The four simultaneous image acquisition channels, usable for reflection or fluorescence, and an
additional transmitted-light channel are ideal for the investigation of multiple fluorescence specimens.
Separately in each of the four channels, the diameters of the pinholes and their XY positions can be
optimized, and the desired emission filter placed into the beam path, by servo-motor control. In the case
of pinhole VP1, this adjustment also includes positioning along Z. In the simultaneous registration of
multiple fluorescences, identical optical sections can be obtained in each confocal channel. This is of
importance, e.g., with the FISH method (fluorescence in-situ hybridization) used for genome analysis in
cytogenetic studies.
The microscope's transmitted-light channel is equipped with a photomultiplier, too. It is therefore
possible to superimpose a multiple fluorescence image on a brightfield, differential interference or phase
image.
A fiber-optic cable connection to external special detectors, such as cooled PMTs or spectrometers, is
under development.
In addition to the emission filters for all standard and special applications, available in motor-controlled
filter wheels, the user can easily install his own emission filters in two of the channels.
The high-NA C-APOCHROMAT objectives specially developed for the LSM technique reach the physical
limit in resolving power, and can be used throughout the 350...700 nm spectral range with the same
high quality, producing brilliant images.
A two-mirror scanner system, controlled by a digital signal processor (DSP), offers several advantages.
The large deflection angle of the scanning mirrors allows a wide area to be scanned. With a 1.25
×
objective, the object area scanned is 10
×
10 mm².
The scanning field size can be freely selected between 4
×
1 and 2048
×
2048 pixels.