General Guidelines for
Superresolution Structured Illumination Microscopy (SR-SIM)
Sample support
In general, we recommend using high-precision coverslips (always no. 1.5 thickness) to mount the samples.
High-precision cover glasses feature an exceptionally accurate thickness of 170 ± 5 µm (= 0,170 ± 0,005 mm).
Also mind thickness issues when ordering glass-bottom petri dishes or multi-well plates.
For cover glasses see:
• ZEISS High precision Cover Glasses 18x18 mm;
ZEISS order Number: 474030-9010-000
• Marienfeld Superior: www.marienfeld-superior.com
• 18x18 mm (Cat.No. 0107032).
• 22x22 mm (Cat.No. 0107052).
• round with 18 mm diameter (Cat.No. 0117580).
• CellPath Ltd UK High performance coverslips
no 1.5H 18x18 mm (Cat.No SAN-5018-03A)
For glass-bottom petri dishes and multi-well plates see:
• Willco (http://www.willcowells.com/)
• Lab-tek (offered through Thermo)
• MatTek (http://glass-bottom-dishes.com)
ZEISS ELYRA
Sample Preparation for Superresolution Microscopy –
a Quick Guide
Authors: Dr. Sylvia Münter
Dr. Yilmaz Niyaz
Carl Zeiss Microscopy GmbH, Germany
Date:
November 2013
White Paper
2
