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Literature References

Reviews on Superresolution Microscopy

1.  G. Patterson et al.: Superresolution imaging using single-molecule localization, Annu Rev Phys Chem 2010 

 

2.  2.  B. Huang et al. Breaking the diffraction barrier: superresolution imaging of cells, Cell 2010

3.  L. Schermelleh et al.: A guide to superresolution fluorescence microscopy, JCB 2010

4.  D. Toomre and J Bewersdorf: A new wave of cellular imaging, Annu. Rev. Cell Dev. Biol. 2010

Superresolution Structured Illumination Microscopy (SR-SIM)

1.  Schermelleh et al.: Subdiffraction imaging of the nuclear periphery with 3D structured illumination microscopy, Science 2008

2.  Gustafsson et al.: Three dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination, 

 

 

Biophys. J. 2008

Fluorescent Proteins for PALM

1.  G. Kremers et al.: Fluorescent proteins at a glance. J Cell Science 2011

2.  G. Patterson: Highlights of the optical highlighter fluorescent protein, J of Microcopy 2011

3.  Shroff et al: Current Protocols in Biology 2010

Sample Preparation and Fluorophore Properties for dSTORM

1.  G.T. Dempsey et al.: Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging 

 

 

Nature Methods 2011 (Performance of fluorophores under different experimental conditions and imaging buffer solutions.)

2.  S. van de Linde et al.: Direct stochastic optical reconstruction microscopy with standard fluorescent probes. 

 

 

Nature Methods 2011. (Detailed overview of fluorophores, experimental conditions and protocols for dSTORM)

Further Reading

Prof. Rainer Heintzmann (http://www.nanoimaging.de)

Prof. Markus Sauer Lab (http://www.super-resolution.biozentrum.uni-wuerzburg.de/) 

ZEISS Online Campus edited by Mike Davidson  (http://www.zeiss.com/campus)

This collection of protocols and information on superresolution sample preparation is just a general guideline. While all attempts  

are made to provide accurate, current and reliable information we cannot guarantee that the protocols will work or that the  

information contained here will be error-free.

White Paper

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Содержание ELYRA

Страница 1: ...ZEISS ELYRA Sample Preparation for Superresolution Microscopy a Quick Guide White Paper...

Страница 2: ...ision Cover Glasses 18x18 mm ZEISS order Number 474030 9010 000 Marienfeld Superior www marienfeld superior com 18x18 mm Cat No 0107032 22x22 mm Cat No 0107052 round with 18 mm diameter Cat No 0117580...

Страница 3: ...or fluorescent proteins are suitable to be used for SR SIM Make sure to have a highly specific labeling with low background to obtain a good signal to noise ratio For multicolor samples the fluoropho...

Страница 4: ...h RI of 1 44 ProLong Gold Life Technologies Be aware this embedding medium needs 2 day to harden in order to reach a constant RI In addition your sample may shrink during this process Refractive index...

Страница 5: ...0 17 14 C All these formats will fit into the adjustable piezo stage insert Please be aware in order to obtain ideal TIRF illumination you need a sufficient mismatch in refractive indices In addition...

Страница 6: ...3 Examples of fluorescent proteins that can be used for PALM imaging Most of these proteins are available through e g Addgene Cambridge MA 02139 USA Recommended FP Pairs for dual color PALM Pair 1 mE...

Страница 7: ...of number of photons per on switching event and the on off duty cycle on STORM image quality for an example structure a ring like object p t and x Images of CCPs clathrin coated pits using Alexa Fluor...

Страница 8: ...Glucose Oxidase e g 24 mg ml GluOx from Aspergillus niger Sigma G0543 50KU 5 l Catalase e g 12 6 mg ml Catalase from Bovine liver Sigma C3155 50 MG Add H2O to final volume of 1ml Very important adjust...

Страница 9: ...to dH2O solution vortex FM solution coat cover slips with Poly L lysine PLL for adhesion of FM add FM Mix solution onto PLL coated cover slips check density 1 3 FM in field of view prepare sample on...

Страница 10: ...ent protein J of Microcopy 2011 3 Shroff et al Current Protocols in Biology 2010 Sample Preparation and Fluorophore Properties for dSTORM 1 G T Dempsey et al Evaluation of fluorophores for optimal per...

Страница 11: ...scopy GmbH 07745 Jena Germany BioSciences microscopy zeiss com www zeiss com elyra EN_41_001_065 CZ 12 2013 Design scope of delivery and technical progress subject to change without notice Carl Zeiss...

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