General Guidelines for Photoactivated Localization Microscopy (PALM)
Sample support
Samples for PALM and dSTORM are ideally prepared on
Nunc Lab-Tek chambers or glass bottom dishes with cover
glass thickness No 1.5.
• Nunc Lab-Tek II Chambered Coverglass no 1.5
(order no.: 155409)
• Greiner Bio-One (order no.: 672860 CELLview)
• Nunc Lab-Tek chambers (order no.:
P35G-0.17-10-C or P35G-0.17-14-C)
All these formats will fit into the adjustable piezo stage
insert.
Please be aware, in order to obtain ideal TIRF illumination
you need a sufficient mismatch in refractive indices.
In addition you may want to change imaging buffer
concentration therefore we recommend no embedding
after fixation of PALM and dSTORM samples.
Note
Nunc Lab-Tek chambers are well suited to transport samples between labs. They fit perfectly into a 50 ml Falcon tube,
that can be filled with PBS and sealed with parafilm.
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Figure 2
ZEISS level adjustable piezo stage insert
