OPERATION
ZEISS
Illumination and contrast methods in transmitted light
Axiolab 5
102
430037-7444-001
05/2019
(3) Configuring the microscope
•
Configure the microscope as for transmitted light brightfield microscopy using the KÖHLER method
(see section 4.2.1 (3)).
•
Center the Pol rotary stage (Fig. 4-13/
1
) (see section 3.1.7.5) and objectives (see section 3.1.7.6).
•
Swing the polarizer (Fig. 4-13/
3
) into the beam path and, if it is rotatable, position it at 0°.
•
Put the analyzer slider (Fig. 4-13/
2
) into the beam path and adjust it using the setting wheel until the
field of view is dark.
•
Bring the specimen to be examined into the field of view and rotate it with the rotary stage. Normally,
birefringent (anisotropic) objects will now show the same color and intensity variations as described
above during rotation between crossed polarizers. Optically anisotropic substances may remain dark
when an isotropic direction, e.g. from optically single-axle or double-axle crystals, is put parallel to the
observation direction.
Fig. 4-13
Components for transmitted light polarization on the conoscopy stand
