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Zeiss Axio Examiner Скачать руководство пользователя страница 61

OPERATION

Axio Examiner

Illumination and contrast procedures

Carl Zeiss

M60-2-0003 e 05/2012

61

4.1.2

Transmitted-light differential interference contrast (DIC)

(1) General operating principle

The transmitted-light DIC procedure is a contrasting alternative for polarization applications, and allows

the high-contrast plastic representation of transparent specimen details.
Light polarized in a linear manner by a polarizer is split into two component beams in a birefringent

prism. These beams run through two neighboring specimen positions located at a small distance from
one another, and are subjected to different path differences brought about by differences in refractive

index and specimen thickness. Subsequently, both component beams are combined in a second
birefringent prism, and have the same oscillation direction after passing through the analyzer. This allows

interference of both component beams in the intermediate image while different path difference are
transposed into different grey values (intensities). A compensator

λ

(lambda plate) converts the grey

values into colors subsequently.

(2) Equipment

−

Objectives offered with DIC equipment, e.g., EC Plan-Neofluars.

−

DIC sliders suitable for the objectives used.

−

Condenser with revolving disk on which DIC prisms are located (DIC I, DIC II, DIC III).

−

Polarizer, e.g., SÉNARMONT polarizer.

−

DIC P&C analyzer module in the reflector turret.

−

Preferably a revolving mechanical stage.

For Axio Examiner microscopes not ordered with DIC equipment, DIC sliders must be inserted into
the objective exchange carriage or into the objective adapter, and set to a central position if any in

order to be able and carry out examinations using differential interference contrast. Step (3)
describes how to insert DIC sliders. Item (4) describes how to set their central position.

(3) Inserting DIC sliders

DIC sliders are already inserted and centered if your microscope equipment has been ordered for

applications using interferential difference contrast.
To retrofit or replace DIC sliders, please proceed as follows:

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Содержание Axio Examiner

Страница 1: ...Operating Manual Axio Examiner Research Microscope...

Страница 2: ...nted Offenders shall be liable for damages All rights reserved for any possible issue of a patent or for utility model registration All company or product designations used in this operating manual ma...

Страница 3: ...ry microscope 28 3 1 5 Installing and removing Push Click modules 29 3 1 6 Installing a reflector insert 31 3 1 7 Installing an objective mount or objective nosepiece 31 3 1 8 Screwing in objectives 3...

Страница 4: ...setting 56 3 4 1 Setting the eyepiece distance interpupillary distance at the binocular tube 56 3 4 2 Setting the viewing height 56 3 4 3 Compensating for eye deficiencies when using eyepiece reticule...

Страница 5: ...ESHOOTING AND SERVICE 82 5 1 Equipment care 82 5 2 Equipment maintenance 82 5 2 1 Checks to be performed 82 5 2 2 Replacing fuses 83 5 3 Trouble shooting 84 5 4 Spares wearing parts and tools 87 5 5 S...

Страница 6: ...ge on the stage carrier 34 Figure 3 15 Setting the ergonomic drive 34 Figure 3 16 Replacing the universal mounting frame 35 Figure 3 17 Attaching the stage carrier stage not shown in the drawing 36 Fi...

Страница 7: ...aminer D1 with DIC equipment 63 Figure 4 6 Adjusting oblique illumination 65 Figure 4 7 Inserting a segmented stop slider 66 Figure 4 8 Components for reflected light fluorescence Axio Examiner A1 68...

Страница 8: ...of Directive 98 79 EC and they are marked with the sign The present operating manual contains information and warnings which are to be observed by the operator The following notices and warning symbo...

Страница 9: ...ffective any longer For restarting the equipment call the Zeiss customer service or the Carl Zeiss Microscopy Service The external power supply unit for 12 V DC 100 W for HAL 100 is designed for using...

Страница 10: ...s not in use Before covering the equipment always check that all lighting devices have been switched off Clogged or covered venting slots may lead to heat buildup which may damage the equipment and ev...

Страница 11: ...o warranty is granted for defects which are either due to natural wear and tear especially for wearing parts or to improper handling The equipment manufacturer shall not be liable for any damage cause...

Страница 12: ...top or bottom part combination selected Legend for Figure 1 2 1 Microscope camera 2 Magnification changer 3 Tube 4 Upper microscope part 5 A 14 x 40 mm stop slider 6 A 14 x 40 mm stop slider or FL att...

Страница 13: ...INTRODUCTION Axio Examiner List of interfaces Carl Zeiss M60 2 0003 e 05 2012 13 Figure 1 2 List of interfaces...

Страница 14: ...ed stop slider 9 Pinion knob for mechanical stage positioning along the Y axis 10 Pinion knob for mechanical stage positioning along the X axis 11 Centering screw for condenser on both sides 12 Filter...

Страница 15: ...INTRODUCTION Axio Examiner Controls and functional elements for Axio Examiner man Carl Zeiss M60 2 0003 e 05 2012 15 Figure 1 3 Controls and functional elements for Axio Examiner man...

Страница 16: ...lider 10 Pinion knob for mechanical stage positioning along the Y axis 11 Pinion knob for mechanical stage positioning along the X axis 12 Centering screw for condenser on both sides 13 Filter wheel 6...

Страница 17: ...INTRODUCTION Axio Examiner Controls and functional elements for Axio Examiner mot Carl Zeiss M60 2 0003 e 05 2012 17 Figure 1 4 Controls and functional elements for Axio Examiner mot...

Страница 18: ...ted light and reflected light microscopes if equipped with a reflected light fluorescence system These microscopes are intended for medical applications to examine tissue samples obtained from the hum...

Страница 19: ...EQUIPMENT DESCRIPTION Axio Examiner System overview Carl Zeiss M60 2 0003 e 05 2012 19 2 2 System overview...

Страница 20: ...EQUIPMENT DESCRIPTION Carl Zeiss System overview Axio Examiner 20 M60 2 0003 e 05 2012...

Страница 21: ...EQUIPMENT DESCRIPTION Axio Examiner System overview Carl Zeiss M60 2 0003 e 05 2012 21...

Страница 22: ...EQUIPMENT DESCRIPTION Carl Zeiss System overview Axio Examiner 22 M60 2 0003 e 05 2012...

Страница 23: ...726 mm x 470 mm Weight Axio Examiner A1 approx 19 kg Axio Examiner D1 approx 21 kg Axio Examiner Z1 incl control box approx 28 kg control box approx 5 5 kg Environmental conditions Transportation in...

Страница 24: ...40 V 10 No line voltage adjustment is required Power frequency 50 to 60 Hz Power consumption for operation with HAL 100 220 VA HBO 100 W power supply unit Field of application indoor Safety class I De...

Страница 25: ...scale interval Maximum stroke range 15 mm Height stop mechanically adjustable Condenser achromatic aplanatic 0 9 H DIC 5x for bright field dark field phase contrast 1 2 3 and DIC Manual objective exc...

Страница 26: ...to read and understand the Equipment Safety Notices carefully before you install the microscope or start using it see page 8 3 1 Installing standard components 3 1 1 Unpacking and installing the micro...

Страница 27: ...3 1 9 3 1 3 Attaching a binocular tube phototube Every binocular tube included in the system overview see section 2 2 may be attached to the Axio Examiner stand Proceed as follows Loosen the hexagon s...

Страница 28: ...Br foc are intended for use together with eyepiece reticules The slight image displacement caused by the additional length of the glass path is taken into account on the diopter scale by the fact that...

Страница 29: ...reflector turret is permanently installed to the Axio Examiner D1 Z1 microscope top parts To either install or remove the modules take off the cap and proceed at an angle from the front side 1 Install...

Страница 30: ...ector insert reflector turret 4x or 6x or reflector slider 2x needs to be taken out of the stand top part 1 Installing a module Take the reflector insert Figure 3 7 1 out of the stand top part see sec...

Страница 31: ...bjective nosepiece To attach the objective mount or objective nosepiece to the stand please proceed as follows For installation move to the lowest Z position possible using the pinion knob Figure 3 9...

Страница 32: ...the WORK position Take the dust caps if any out of the corresponding openings at the objective mount or objective nosepiece Take the objective s Figure 3 11 3 out of their container and screw them int...

Страница 33: ...r Hold the condenser carrier Figure 3 12 2 with one hand and unscrew the two hexagon socket screws Figure 3 12 1 Take off the condenser carrier and lay it down safely 3 1 10 Installing the condenser T...

Страница 34: ...the drive length for X and Y axis positioning may be increased by a maximum of 15 mm by displacing the pinion knobs in axial direction Removing or installing additional 3 1 11 2 sleeves Both pinion k...

Страница 35: ...Y pinion knob Figure 3 15 7 in position and turn the light colored knurled sleeve Figure 3 15 8 located above either to the right easy or to the left heavy until the desired easy motion is obtained Sl...

Страница 36: ...17 2 slightly with your right hand Align the stage carrier correspondingly at the lower stop screw and tighten the clamping screw Figure 3 17 2 without using excessive pressure If the adapter is used...

Страница 37: ...l guide Figure 3 17 1 at the right hand side Then remove the stage carrier from the stand by pulling it toward the front and lay it down on the table preventing it from overturning 3 1 13 HAL 100 halo...

Страница 38: ...en set the adjusting screws Figure 3 19 4 and 5 to a value at which the lamp filaments of one image exactly fill out the gaps in the reflector image Figure 3 19 1 2 Fine adjustment Attach the microsco...

Страница 39: ...ctor Push down the unlock key Figure 3 20 3 of the HAL 100 halogen lamp Figure 3 20 1 pull out the bulb holder Figure 3 20 2 completely and put it down separately Push down both spring levers Figure 3...

Страница 40: ...separately Press the spring lever Figure 3 22 5 at the heat sink pull the heat sink Figure 3 22 1 upwards and off the inserted dummy or off the used burner Figure 3 22 2 and lay it down carefully Mak...

Страница 41: ...ing shown at the hours run meter transformer Replace the burner when it has reached its nominal useful life of 100 hours Attaching the HBO 50 illuminator 3 1 14 2 Attach and align the HBO 50 illuminat...

Страница 42: ...e in a centered location in parallel to one another within the adjusting circle in the observation window Figure 3 24 5 Electric arc and mirror image should not be superimposed one on top of the other...

Страница 43: ...hanging transmission unless permanently installed Neutral density filters will not last in the long run Attaching the HBO 100 3 1 15 2 illuminator Remove the cap from the reflected light socket Figure...

Страница 44: ...omewhat darker mirror image will appear in the adjusting aid s black glass window Turn the knurled knob Figure 3 28 4 to focus the brighter focal spot using the collector adjustment Turn the adjusting...

Страница 45: ...rner mirror image by analogy to the focal spot image Figure 3 27 2 within the inscribed adjusting circle The two focal spots of the HBO 103 W 2 lamp should be located close to one another within the a...

Страница 46: ...unit 3 2 1 Connecting the Axio Examiner mot In the Axio Examiner mot lower microscope part 430031 9010 000 focusing is insured exclusively by the motor driven Z axis positioning of the objective moun...

Страница 47: ...ner control unit 3 2 2 Connecting and switching the HAL 100 halogen lamp on or off The HAL 100 halogen lamp is powered and switched either on or off by the external power supply unit The external powe...

Страница 48: ...of the HBO 100 W transformer and secure it by a retaining ring Connect the HBO 100 W transformer s AC power socket Figure 3 31 3 to a mains outlet Use the power switch Figure 3 31 1 to switch the HBO...

Страница 49: ...to the assembly opening located at the front side and use it to lift off the cover Hold the stand top part Figure 3 32 4 in position screw out the six hexagon socket head screws Figure 3 32 2 and remo...

Страница 50: ...33 3 Put on the cap with the opening Figure 3 33 2 Slide in the slidable adjusting aid socket 3 3 3 Installing an adapter for 45 mm objectives Only for Axio Examiner D1 Z1 Use the shift knob Figure 3...

Страница 51: ...ter set in the FL P C reflector module The filter sets for the FL P C reflector module may be combined and installed on an individual basis by the customer Corresponding filter sets or completely asse...

Страница 52: ...Figure 3 38 2 and spring frame Figure 3 38 3 out of the lower half module Remove the old beam splitter and position the new beam splitter with the reflecting side pointing down onto the spring frame F...

Страница 53: ...the guide of the mounting frame Attach condenser and stage carrier again 3 3 8 Replacing the DIC prism in the condenser To take out the cover plate turn the condenser cover plate Figure 3 40 4 using t...

Страница 54: ...d Figure 3 41 2 up and out of the corresponding position of the filter wheel Figure 3 41 3 using a pair of tweezers Insert a new filter into the filter wheel Figure 3 41 3 A filter position is always...

Страница 55: ...rim must fit in the slot at the condenser Run the discharge hose Figure 3 42 2 to a corresponding vessel dish etc Put on the stage carrier see section 3 1 12 3 3 11 Attaching a condenser carrier with...

Страница 56: ...iece reticules The correct use of an eyepiece reticule requires two adjustable eyepieces e g PL 10x 23 Br foc for compensating different eye deficiencies of a viewer Use the focusable eyepiece lens of...

Страница 57: ...lock in resistance is set Insert the closing plug again Figure 3 46 1 Put on the binocular tube see section 3 1 3 3 4 5 Setting the actuating rod Only for phototubes 425521 9020 000 and 425521 9000 00...

Страница 58: ...oscopy procedures since it allows the quick and easy viewing of high contrast or stained specimens e g blood films In addition to so called direct beams indirect ray beams i e beams diffracted and sca...

Страница 59: ...ut of position by the condenser Switch the revolving disk of the condenser where existing using the knurled ring Figure 4 2 4 to the H position for the bright field Swing the 10x objective in position...

Страница 60: ...nge changes both the visual field size and the objective aperture This requires a new setting of bright field and aperture stop in order to achieve optimum results If objectives 10x are used the apert...

Страница 61: ...ermediate image while different path difference are transposed into different grey values intensities A compensator lambda plate converts the grey values into colors subsequently 2 Equipment Objective...

Страница 62: ...locks exactly into position Axio Examiner D1 Z1 with W Plan Apochromat 20x 1 0 objective 75 mm parfocal length Insert DIC slider S NARMONT 75 W PA 426967 9000 000 or 426967 9070 000 Figure 4 4 1 into...

Страница 63: ...e 10x objective or the overview objective used Figure 4 5 4 is in working position The condenser turret has been swiveled into the bright field or into an unequipped position Swing the S NARMONT polar...

Страница 64: ...revolving disk The condenser used must not be equipped with DIC prisms which are installed in combination with polarizing filters Switch on the S NARMONT polarizer Figure 4 5 2 polarizer and 4 plate i...

Страница 65: ...edure HAL 100 halogen lamp for transmitted light illumination The following condensers may be used for oblique illumination Condenser 0 8 424226 9000 000 Condenser 0 9 424226 9010 000 Condenser 1 2 42...

Страница 66: ...rticular for highly scattering tissue sections 2 Equipment The equipment of every microscope allows the performance of the oblique illumination procedure All condensers available for delivery may be u...

Страница 67: ...litter Finally the beams pass a barrier filter long pass band pass filter which only allows the passage of the long wave radiation emitted by the specimen Excitation filter and barrier filter require...

Страница 68: ...itation at the reflector turret Figure 4 8 6 Enable the optical path in the reflected light illuminator when using the reflected light filter drawer Figure 4 8 2 If required set the illumination inten...

Страница 69: ...inocular phototube Actuating rod Actuating rod pushed in Visual examination through the eyepieces Actuating rod pulled out Camera output enabled Shift knob Turn the shift knob Figure 1 3 3 to toggle b...

Страница 70: ...see Figure 4 9 Navigation bar 4 3 1 1 The navigation bar is located at the left hand screen side and may be used to open all pages The keys available depend on the current page But the following keys...

Страница 71: ...keys work like switches 3 Operator control This area contains control elements which depend on the button selected either in the navigation bar or in the tab 4 Popup windows Popup windows will appear...

Страница 72: ...Info Extras Components XY Z Soft keys Position Focus Mode Buttons Left Buttons Right Stand type Language Ethernet Misc Reflector Cond Focus Navigation bar 2nd level Reflector Misc Firmware Figure 4 11...

Страница 73: ...l motors or sensors of the microscope either on or off Motors and sensors will be grounded earthed potential free when switched off and the blue indicator light Figure 1 4 19 will go off at the lower...

Страница 74: ...The two Set zero buttons work as follows man Set the zero point manually i e the current position is defined as the zero point and the display is set to zero auto Set the zero point automatically i e...

Страница 75: ...te positions see 2 Save position above 4 3 6 Settings Press the Settings button in the navigation bar on the Home page to go to the Settings page From the Settings page you can go to the Components Us...

Страница 76: ...b to set the focusing gear speed compensate objective parfocality or switch automatic parfocality compensation either on or off 3 Misc ellaneous This tab enables the operator to configure additional o...

Страница 77: ...12345 2 Buttons left To modify the button assignments the operator needs to type in an administrator password Otherwise the operator may only view the button assignments without being able to customi...

Страница 78: ...6 4 The Settings About page only contains the Firmware tab This tab indicates the firmware version to the operator 4 3 7 Display Press the Display button in the navigation bar on the Home page to ope...

Страница 79: ...ay be written on the adhesive labels supplied The labels may be glued to the fields provided 4 4 2 Axio Examiner Z1 upper microscope part with sideport The Axio Examiner Z1 upper microscope part with...

Страница 80: ...To enable the click stop again rotate the adjusting screw Figure 4 21 3 by 90 in the opposite direction 2 Adjusting the cameras to one another If two cameras are used they need to be adjusted to one...

Страница 81: ...s M60 2 0003 e 05 2012 81 Unscrew the knurled screw Figure 4 22 1 at the camera port on the rear side To adjust the camera in X and Y direction turn the two adjusting screws width A F 1 5 Figure 4 22...

Страница 82: ...the center to the rim using a slightly moistened swab Remove dust from optical surfaces using a natural hair brush or blow it off using a rubber ball air blower Please observe the following when you...

Страница 83: ...inlet and contains two fuses of the following types T 5 0 A H 5x20mm Examiner control and 12 V DC 100 W external power supply unit or T 2 0 H A HBO 100 transformer or T 4 A mbq52ac z 100 V 127 V trans...

Страница 84: ...correctly centering opening see page 58 and the following Filter not placed correctly into the filter holder Place filter correctly into the filter holder Low resolution and bad image contrast Apertur...

Страница 85: ...distance not set correctly at the binocular tube Adjust the eyepiece distance correctly see page 56 Focusable eyepieces are not adjusted correctly Adjust the focusable eyepieces to the eye deficiencie...

Страница 86: ...halogen lamp is flickering Its luminosity is not stable The 12 V 100 W halogen lamp is at the end of its mean service life Replace the 12 V 100 W halogen lamp see page 39 AC power cord not installed...

Страница 87: ...screwdriver width A F 2 5 Ball head screwdriver width A F 1 5 For setting oblique illumination at the condenser Eyepiece eyecup Recommendable for reflected light suppression during low luminosity proc...

Страница 88: ...service or by especially authorized expert personnel To ensure the optimum setting and trouble free operation of your microscope for a long period of time we recommend to sign a service or maintenance...

Страница 89: ...ards Institution DL Transmitted light DX Coding system for storing electronically readable information e g film speed EG European Community EN Euronorm EMC Electromagnetic compatibility ENG Electronic...

Страница 90: ...across flats T Slow blow a fuse type TV Television T2 Adapter Standardized adapter for miniature cameras UL Underwriter Laboratories UV Ultraviolet VDE Verband Deutscher Elektrotechniker Association...

Страница 91: ...ce 82 Click stop 80 Coarse motion 14 16 Colibri 45 Condenser 14 16 33 53 55 59 Height adjustment 14 16 Condenser carrier 33 motor operated 55 Conversion 49 D DIC 61 DIC condenser module 53 DIC slider...

Страница 92: ...45 I Illumination and contrast procedures 58 Illuminator HBO 100 44 Illuminator HBO 50 40 Infrared 14 16 Installing 26 IR 60 K Key ring 16 K HLER 58 L Light sources 24 Line voltage 24 25 Lock in resi...

Страница 93: ...minator 68 Reflector insert 31 Reflector module 52 Reflector turret 16 25 57 79 Reflector turret mot 46 S Segmented stop 14 16 Segmented stop slider 14 16 S NARMONT polarizer 53 63 Service 88 Shift kn...

Страница 94: ...APPENDIX Carl Zeiss Subject index Axio Examiner 94 M60 2 0003 e 05 2012 V Viewing height 56 VIS 14 16 60 W Warning signs 11 Warranty instructions 11 Wearing parts 87 Weight 23...

Страница 95: ...DIX Axio Examiner Industrial property rights Carl Zeiss M60 2 0003 e 05 2012 95 6 3 Industrial property rights The equipment equipment parts or procedures described in this manual are protected by pat...

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