YL Instruments YL9100 HPLC, Руководство пользователя

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YL9111 Binary Pump
method, and be careful when treating strong acid and strong base.
1) Separate column inlet tubing connected to column.
2) Orient column inlet tubing toward waste bottle.
3) Set flow rate at 1ml/min.
4) If injector is installed, turn it to injection position.
5) Pump 100% iso-propanol through pump and injector for 10 minutes.
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6) Pump distilled water filtered through pump and injector for 10 minutes.
7) Pump 10% nitric acid solution for 5 minutes.
8) Wash pump and injector with distilled water filtered for at least 10 minutes.
9) Pump 100% iso-propanol through pump and injector for 5 minutes.
Now, pump is prepared for use of mobile phase or for the period not being used for short time or long
time. If pump is not used for long time or there is contamination in flow path due to use of impure solvent,
it is desired to separate pump head assembly and wash it with ultrasonic cleaner. In order to wash pump
head, separate pump head into parts in the same manner as seal change process of 5.2, wash it with
ultrasonic cleaner, and assemble each part again. At this time, the high pressure seal is damaged, so
replace it with new one.
5-7. Supply of lubricant
YL9111 Binary pump necessitates supply of proper lubricant into piston drive part for smooth operation
of instrument. It is desired to use lubricant or low viscosity grease for piston carrier and pump housing
and small amount of grease such as 630-AA for bearing of cam shaft and piston carrier. Care should be
taken because pumping action is interfered with if lubricant is attached to surface of piston. Because
shortening of pump life is caused where powder or dust is much generated, install instrument where
surrounding environment is good.
5-8. Replacement of solvent filter and in-line filter
In case instrument is used for long time or mobile phase is bad, mobile phase filter and in-line filter is
blocked due to small particles contained in solvent. If filter is blocked, pressure within flow path of pump is
largely reduced when solvent is sucked to generate air bubble, make flow rate reduced and make
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