WPI STX2-PLUS, Инструкция по эксплуатации

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STX2-PLUS
World Precision Instruments 3
OPERATING INSTRUCTIONS
Positioning Electrode in a Culture Cup
Proper placement of the electrode in the cell is critical to making accurate
measurements. The STX2-PLUS electrode is designed to facilitate measurements of
membrane voltage and resistance of cultured epithelia
in tissue culture wells. The lengths of the electrodes
are unequal allowing the longer (external) electrode to
touch the bottom of the dish containing the external
culture media while preventing the shorter (internal
electrode) from reaching the bottom of the tissue
culture cup or insert (see Fig. 1) below). This feature
ensures proper repeatable positioning between the
electrode and the cell layer in the cup during the trans
membrane measurement. The reproducibility of the
measurements is significantly improved.
Fig. 2— (Right) Electrodes in solution.
Stable Repeatable Readings
This new electrode design sets the correct depth and centering of the electrode
elements. Once placed and released, movement ceases, the EVOM3 readings become
more stable, negating any need to make multiple measurements.
Ease of Use
The STX2-PLUS is designed for use with Corning 347X 24-well plates or Millipore
hanging inserts. The apical volume is 250 µL, and the basal volume is 750 to 1000 µL.
Insert the outer electrode into the basal access, center the lower adjustment ring onto
the upper edge of the insert and let the electrode stand on its own. The electrode legs
no longer need to flex or be inserted at an angle (Fig. 4). The electrode placement is
stable and repeatable.
*For 24-well HTS plates, keep the basal electrode centered in the basal access.
*For 12-well inserts, the
STX2-PLUS must be hand held.
Fig. 3—Correct Fig. 4—Angle variance Fig. 5—Depth variance