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STX2-PLUS

World Precision Instruments 

7

Testing the STX2-PLUS Electrodes

If another electrode is being used, refer to that electrode’s manual.

To make sure the electrode is operating properly, a resistance test in KCl is used. This 

test demonstrates electrode functionality.

40 mM KCl

80 mM KCl

160 mM KCl

38-66 Ω

27–44 Ω

12–20 Ω

At the lower molarities (10–20 mM), a DMEM coated electrode will begin to show 

instabilities at the higher concentrations. If the resistance readings in the KCl mixtures 

are over 10% out of range, then the electrode may need cleaning. See “Cleaning/

Maintaining the STX2-PLUS Electrodes” on page 5. An Excel scatter plot of the 

molarity vs. resistance (as a power) should show an R

2

 value > 0.99x.

NOTE

: These resistance readings can vary due to fluid volume and electrode depth. 

These values are a reference. An Excel scatter plot of the molarity vs. resistance 

(as a power) should show an R

2

 value > 0.99x. If the R

2

 value is acceptable, then the 

electrode can be used even if it is slightly out of range. If the resistance readings in the 

KCl mixtures are out of range, then the electrode may need cleaning. See “Cleaning/

Maintaining the STX2-PLUS Electrodes” on page 5.  Electrodes which are not being 

properly maintained may begin to show instabilities indicating the need for cleaning. 

Regular maintenance of the electrode is strongly recommended for longevity. 

ACCESSORIES

99675

 

EVOM2 Adaptor Cable

7363-4

 

Enzol Enzymatic Detergent

Fig. 10—Plug the STX2-PLUS into the 99675 cable to use the electrode with an EVOM2. The 

99675 cable is sold separately. 

Содержание STX2-PLUS

Страница 1: ...WORLD PRECISION INSTRUMENTS STX2 PLUS for use with EVOM3 Epithelial Voltohmmeter Serial No _____________________ www wpiinc com INSTRUCTION MANUAL 082620...

Страница 2: ......

Страница 3: ...NG INSTRUCTIONS 3 Positioning Electrode in a Culture Cup 3 Stable Repeatable Readings 3 Ease of Use 3 Adjusting the Length of the Electrodes 4 MAINTENANCE 5 Cleaning Maintaining the STX2 PLUS Electrod...

Страница 4: ...ii World Precision Instruments...

Страница 5: ...rectly in 12 and 24 well tissue culture plates The electrode incorporates a adjustable pair of probes 5 mm tall 4 mm wide and 0 7 mm in thickness Each probe has an outer current and an inner voltage e...

Страница 6: ...any parts are missing at 941 371 1003 or customerservice wpiinc com Returns Do not return any goods to WPI without obtaining prior approval RMA required and instructions from WPI s Returns Department...

Страница 7: ...ility of the measurements is significantly improved Fig 2 Right Electrodes in solution Stable Repeatable Readings This new electrode design sets the correct depth and centering of the electrode elemen...

Страница 8: ...a hanging insert Fig 6 Rotate the nut to adjust the length of the electrodes Fig 7 The electrode length is properly adjusted 2 Align the electrodes with the well inserts and position the STX2 PLUS ove...

Страница 9: ...to your maintenance schedule and the frequency of your cleaning Soak the electrodes overnight when the electrodes have not been on a routine maintenance cleaning schedule Soak 60 minutes if you are o...

Страница 10: ...us electrode must be dipped into chlorine bleach 5 sodium hypochlorite on occasion to maintain the amount of Ag to AgCl balance Otherwise the electrode will become unstable A 10 15 minute immersion of...

Страница 11: ...should show an R2 value 0 99x NOTE These resistance readings can vary due to fluid volume and electrode depth These values are a reference An Excel scatter plot of the molarity vs resistance as a pow...

Страница 12: ...ng Radio frequency interference Turn off or move any cellular phones farther away from the experimental setup NOTE If you have a problem issue with that falls outside the definitions of this troublesh...

Страница 13: ...25 mL of 20 mM KCl 25 mL 50 mL 10 mM KCl APPENDIX B COMPATIBLE INSERTS AND PLATES Corning Millipore Material Membrane Diameter mm Growth Surface Area cm2 Membrane Pore Size m 3470 6 5 0 33 0 4 3472 PI...

Страница 14: ...10 World Precision Instruments Millicell Membrane Pore Size m Qty Pkg MCHT24H48 0 4 48 MCRP24H48 1 0 48 MCSP24H48 3 0 48 MCMP24H48 5 0 48 MCEP24H49 8 0 48...

Страница 15: ...special or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or i...

Страница 16: ...Tel 49 0 6031 67708 0 Fax 49 0 6031 67708 80 E mail wpide wpi europe com China Hong Kong WPI Shanghai Trading Co Ltd Rm 27A Liangfeng Building No 8 Dongfang Road Pudong New District Shanghai PR China...

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