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STX2-PLUS

World Precision Instruments 

5

MAINTENANCE

Electrodes must be properly cleaned, sterilized and stored.

CAUTION

: Do not flame electrodes. Doing so will cause them to melt. 

Cleaning/Maintaining the STX2-PLUS Electrodes

IMPORTANT! 

With the use of DMEM, the electrode surface will become coated with 

protein or other foreign materials. This build-up, or contamination, will degrade the 

performance of the system. After every use, rinse the STX electrodes with distilled 

water and store them dry. Periodically clean your STX electrodes with Tergazyme, 

Enzol or Endozyme proteolytic detergents. (These detergents are manufactured by 

Alconox, Johnson & Johnson, Ruhof.)
1.  Rinse with the electrodes with distilled water and dry them.
2.  Make a 1% solution of Tergazyme according to the manufacturer’s instructions.
3.  Suspend the tips of the electrodes in the Tergazyme solution, with the exposed 

electrode surfaces fully immersed. During soaking, you can brush the surfaces 

of the electrodes with a soft brush (like a toothbrush), if desired. The soaking 

time varies according to your maintenance schedule and the frequency of your 

cleaning.

• 

Soak the electrodes overnight when the electrodes have not been on a 

routine maintenance cleaning schedule.

• 

Soak 60 minutes if you are on a weekly cleaning schedule.

• 

Soak 10-30 minutes if you clean your electrodes daily.

4.  Rinse the electrodes well with distilled or de-ionized water. Allow them to air dry 

and store the electrodes dry away from exposure to sunlight.

Disinfecting the STX2-PLUS Electrodes

The 

STX2-PLUS

 electrodes are resistant to most methods of low temperature 

chemical disinfection. A solution of 5% sodium hypochlorite (undiluted household 

bleach) is a good choice. Ortho-phthaladehyde (Cidex OPA or Rapicide OPA), 30/70 

ethanol or 30/70 isopropyl alcohol are also acceptable.
The 

STX2-PLUS

 electrodes require a 10-15 minute soak in a solution of 5% sodium 

hypochlorite (undiluted household bleach) to revitalize the electrodes.

CAUTION

: NEVER leave the electrode in alcohol for more than 30 minutes at a 

time. Continuously soaking the electrode in alcohol will weaken the protective 

coating on the electrode and shorten its life. A damaged electrode showing 

peeling of the transparent coating will expose tissues to copper. An electrode showing 

signs of peeling must be replaced.

Содержание STX2-PLUS

Страница 1: ...WORLD PRECISION INSTRUMENTS STX2 PLUS for use with EVOM3 Epithelial Voltohmmeter Serial No _____________________ www wpiinc com INSTRUCTION MANUAL 082620...

Страница 2: ......

Страница 3: ...NG INSTRUCTIONS 3 Positioning Electrode in a Culture Cup 3 Stable Repeatable Readings 3 Ease of Use 3 Adjusting the Length of the Electrodes 4 MAINTENANCE 5 Cleaning Maintaining the STX2 PLUS Electrod...

Страница 4: ...ii World Precision Instruments...

Страница 5: ...rectly in 12 and 24 well tissue culture plates The electrode incorporates a adjustable pair of probes 5 mm tall 4 mm wide and 0 7 mm in thickness Each probe has an outer current and an inner voltage e...

Страница 6: ...any parts are missing at 941 371 1003 or customerservice wpiinc com Returns Do not return any goods to WPI without obtaining prior approval RMA required and instructions from WPI s Returns Department...

Страница 7: ...ility of the measurements is significantly improved Fig 2 Right Electrodes in solution Stable Repeatable Readings This new electrode design sets the correct depth and centering of the electrode elemen...

Страница 8: ...a hanging insert Fig 6 Rotate the nut to adjust the length of the electrodes Fig 7 The electrode length is properly adjusted 2 Align the electrodes with the well inserts and position the STX2 PLUS ove...

Страница 9: ...to your maintenance schedule and the frequency of your cleaning Soak the electrodes overnight when the electrodes have not been on a routine maintenance cleaning schedule Soak 60 minutes if you are o...

Страница 10: ...us electrode must be dipped into chlorine bleach 5 sodium hypochlorite on occasion to maintain the amount of Ag to AgCl balance Otherwise the electrode will become unstable A 10 15 minute immersion of...

Страница 11: ...should show an R2 value 0 99x NOTE These resistance readings can vary due to fluid volume and electrode depth These values are a reference An Excel scatter plot of the molarity vs resistance as a pow...

Страница 12: ...ng Radio frequency interference Turn off or move any cellular phones farther away from the experimental setup NOTE If you have a problem issue with that falls outside the definitions of this troublesh...

Страница 13: ...25 mL of 20 mM KCl 25 mL 50 mL 10 mM KCl APPENDIX B COMPATIBLE INSERTS AND PLATES Corning Millipore Material Membrane Diameter mm Growth Surface Area cm2 Membrane Pore Size m 3470 6 5 0 33 0 4 3472 PI...

Страница 14: ...10 World Precision Instruments Millicell Membrane Pore Size m Qty Pkg MCHT24H48 0 4 48 MCRP24H48 1 0 48 MCSP24H48 3 0 48 MCMP24H48 5 0 48 MCEP24H49 8 0 48...

Страница 15: ...special or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or i...

Страница 16: ...Tel 49 0 6031 67708 0 Fax 49 0 6031 67708 80 E mail wpide wpi europe com China Hong Kong WPI Shanghai Trading Co Ltd Rm 27A Liangfeng Building No 8 Dongfang Road Pudong New District Shanghai PR China...

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