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World Precision Instruments

CAUTION

: Ammonia is NOT recommended, because silver chloride dissolves 

in ammonia.

Sterilizing the STX2-PLUS Electrode

The 

STX2-PLUS

 electrodes are non-sterile as supplied. Acceptable low temperature steril-

ization methods for the electrodes include gamma irradiation and ethylene oxide gas (ETO).  

CAUTION

: Do NOT autoclave the electrode. 

CAUTION

: Do NOT use ionized hydrogen peroxide (H

2

O

2

). 

CAUTION

: Do NOT expose the electrodes to ultra-violet light, because UV light 

decomposes silver/silver chloride electrodes. 

CAUTION

: Do not sand or abrade the electrode surfaces. A damaged 

electrode will expose tissues to copper. An electrode showing deep scrateches 

on the Ag-AgCl surfaces must be replaced.

CAUTION

: Do not immerse the electrode head.  

Rechloriding the Electrodes

The 

STX2-Plus

 electrode must be dipped into chlorine bleach (5% sodium 

hypochlorite) on occasion to maintain the amount of Ag to AgCl balance. Otherwise, 

the electrode will become unstable. A 10–15 minute immersion of the tips is 

recommended before each daily use. (These treatments may be reduced to once 

per week as experience guides). DMEM deposits cause resistance measurements to 

become unstable and the coating appears as a dark crust on the electrode surfaces. 

See “Appendix A: KCL testing mixtures” on page 9. A properly chlorided silver 

surface will appear to be a dark silvery grey coloration.
https://www.wpiinc.com/wp-34521-endozime-enzymatic-detergent-169-oz 

https://www.wpiinc.com/7363-4-enzol-enzymatic-detergent-1-gallon

CAUTION

: Too much chloriding can also lead to instabilities. A short 5 minute 

soak in ammonia water (3%) will convert the AgCl back to Ag. After a DI water 

rinse, repeat the chloriding. Follow this with an electrode test.

Storing the Electrodes

After a daily use, wash and store the electrodes dry.

Содержание STX2-PLUS

Страница 1: ...WORLD PRECISION INSTRUMENTS STX2 PLUS for use with EVOM3 Epithelial Voltohmmeter Serial No _____________________ www wpiinc com INSTRUCTION MANUAL 082620...

Страница 2: ......

Страница 3: ...NG INSTRUCTIONS 3 Positioning Electrode in a Culture Cup 3 Stable Repeatable Readings 3 Ease of Use 3 Adjusting the Length of the Electrodes 4 MAINTENANCE 5 Cleaning Maintaining the STX2 PLUS Electrod...

Страница 4: ...ii World Precision Instruments...

Страница 5: ...rectly in 12 and 24 well tissue culture plates The electrode incorporates a adjustable pair of probes 5 mm tall 4 mm wide and 0 7 mm in thickness Each probe has an outer current and an inner voltage e...

Страница 6: ...any parts are missing at 941 371 1003 or customerservice wpiinc com Returns Do not return any goods to WPI without obtaining prior approval RMA required and instructions from WPI s Returns Department...

Страница 7: ...ility of the measurements is significantly improved Fig 2 Right Electrodes in solution Stable Repeatable Readings This new electrode design sets the correct depth and centering of the electrode elemen...

Страница 8: ...a hanging insert Fig 6 Rotate the nut to adjust the length of the electrodes Fig 7 The electrode length is properly adjusted 2 Align the electrodes with the well inserts and position the STX2 PLUS ove...

Страница 9: ...to your maintenance schedule and the frequency of your cleaning Soak the electrodes overnight when the electrodes have not been on a routine maintenance cleaning schedule Soak 60 minutes if you are o...

Страница 10: ...us electrode must be dipped into chlorine bleach 5 sodium hypochlorite on occasion to maintain the amount of Ag to AgCl balance Otherwise the electrode will become unstable A 10 15 minute immersion of...

Страница 11: ...should show an R2 value 0 99x NOTE These resistance readings can vary due to fluid volume and electrode depth These values are a reference An Excel scatter plot of the molarity vs resistance as a pow...

Страница 12: ...ng Radio frequency interference Turn off or move any cellular phones farther away from the experimental setup NOTE If you have a problem issue with that falls outside the definitions of this troublesh...

Страница 13: ...25 mL of 20 mM KCl 25 mL 50 mL 10 mM KCl APPENDIX B COMPATIBLE INSERTS AND PLATES Corning Millipore Material Membrane Diameter mm Growth Surface Area cm2 Membrane Pore Size m 3470 6 5 0 33 0 4 3472 PI...

Страница 14: ...10 World Precision Instruments Millicell Membrane Pore Size m Qty Pkg MCHT24H48 0 4 48 MCRP24H48 1 0 48 MCSP24H48 3 0 48 MCMP24H48 5 0 48 MCEP24H49 8 0 48...

Страница 15: ...special or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or i...

Страница 16: ...Tel 49 0 6031 67708 0 Fax 49 0 6031 67708 80 E mail wpide wpi europe com China Hong Kong WPI Shanghai Trading Co Ltd Rm 27A Liangfeng Building No 8 Dongfang Road Pudong New District Shanghai PR China...

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