WPI MPS-4, Инструкция по эксплуатации, Страница: 17 / 36

MPS-4
World Precision Instruments 13
MAINTENANCE
Cleaning
Clean the tubing system before and after each experiment. Although the solution path
of the solenoid valve in MPS-4 does not contact with any metal parts, but the drugs
remaining in the solenoid valve will affect the accuracy of subsequent experiments.
So at the end of each experiment, the valve needs to be flushed with distilled water.
Drain the water out afterwards. The protocols for cleaning are as follows:
1. Remove the perfusion µ-manifold from the holding rod.
2. Carefully remove each of the 8 pieces of tubing from the adaptors on the back
of the manifold. It is best to push them from their ends since pulling them off
may damage the perfusion manifold. Pressurizing the tubing may facilitate this
procedure.
3. Turn on the control switches and discharge the drug solutions from all 8 tubing
channels.
4. Keeping the switches open, fill each syringe with warm, distilled water to wash the
tubing and valves. Repeat this step 2 to 3 times.
5. After the manifold is removed from the tubing (step 2), press the provided
cleaning adaptor onto the manifold from the tip end. Connect it to a syringe filled
with filtered water and flush it.
CAUTION : Unfiltered water could clog the manifold and permanently damage
it. The manifold is made of PMMA material. It can only be washed with water.
Any organic solvent, even alcohol, can permanently damage it. If alcohol must
be used, only use ethyl alcohol.
TROUBLESHOOTING
If there is no perfusion, check the following to locate the problem:
1. You can tell if the perfusion controller is running a perfusion sequence by looking
at the lights above the numbered manual control buttons. When a valve is being
opened, the corresponding light should turn on. If the perfusion controller won’t
turn on at all, check the power cord and the fuse in the back panel. If there is
trouble communicating with the computer in Online mode, make sure the serial
cable is tight and try restarting both the instrument and the PC software.
2. The lights above each channel of the valve console turn on when the valve is
opened. In addition, there a soft click when a valve is turned on or off. If the valve
console is not responding, tighten the cable that connects it to the perfusion
controller.
3. Make sure the stopcock is in the correct position. The middle protrusion on the
knob should be facing away from the syringe fluid port on the side.
4. Visually check for air bubbles or obstructions in any segments of tubing. Test
the µ-manifold by connecting a syringe directly to one of its input passages