WPI EZ Anesthesia, Инструкция по эксплуатации

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EZ Anesthesia
World Precision Instruments 7
your own lab testing. Factors specific to your laboratory context may impact protocols.
Protocols should be developed based on your requirements and careful observation
of animals during anesthesia. Check the reflexes of the animal before transferring
them to the surgical table.
Prior to Operation
WARNING: THE SYSTEM MUST BE PROPERLY SET UP BEFORE TURNING
ON ANY GAS.
NEVER disconnect any hoses until the oxygen has been shut off and the vaporizer
is shut off. Failure to precisely follow these instructions could result in injury. Back
pressure can cause the vaporizer hoses to pop, and it can cause Isoflurane to backup
and leak into the manifold and possibly the flowmeter, therefore affecting system
performance and accuracy.
1. Verify that all tubes are properly connected.
2. Check that water pump is operating and surgical bed is at proper temperature.
WARNING: IMPORTANT! MAKE SURE THE SMALL BLACK PLUG IS
INSERTED INTO BREATHING DEVICE NOSECONE. THE OPERATOR
COULD FACE EXPOSURE TO ISOFLURANE IF THE PLUG IS NOT INSERTED
PROPERLY.
3. Check that the inlet flowmeter on the vaporizer stand is fully closed by turning it
clockwise.
4. Connect the desired components to be used with the system (for example,
breathing devices and chamber). At least one component must be connected
before turning on any gas.
5. If the induction chamber is to be used for initial anesthesia, verify that the
secondary flow meter is turned fully counterclockwise to open.
7. Fully open the oxygen tank valve.
8. Turn the inlet flowmeter knob counterclockwise to open and adjust it to:
• Mice: 1 liter/min.
• Rats: 1.5 liter/min.
• Guinea Pigs: 3 liter/min.
9. If you are using the induction chamber, check for oxygen flow through the
secondary flowmeter. The secondary flow meter should read between 1-3 liters/
Min. depending on the species.
Induction
1. Place animals into the induction chamber and latch the lid.