WPI APOLLO 4000, Инструкция по эксплуатации

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APOLLO 4000
OXYGEN DETECTION
World Precision instruments d-0
less than the length of the sleeve, so that it will not puncture the delicate
membrane at the tip of the sleeve during injection. if the microFil is longer
than the sleeve it can be cut to the correct length.
8. slowly and smoothly insert the electrode into the sleeve, and screw the
locking cap into the handle. the electrode should be observed to press
gently against the membrane.
9. the current at this time will be high or offscale.
10. suspend the tip of the new assembled probe in PBs buffer solution.
11. After 0-5 minutes the current should no longer be offscale and will
gradually decrease with time. it may take several hours for the sensor
current to reach a low stable value, at which time it will be ready for use.
12. if the current observed, after a few minutes in the saline solution,
increases dramatically or is offscale, then the membrane integrity is not
good and a new membrane will have to be fitted.
13. When the iso-oXY-2 is not being used it should be stored with the tip
suspended in distilled water.
Clean the Membrane
the membrane sleeve itself requires very little maintenance. the primary concern
is to avoid to damage the membrane and to keep it as clean as possible. After
each use, the membrane should be cleaned by suspending the tip in distilled
water for 20-30 minutes to dissolve salts and remove particles ,which may
have accumulated on the membrane. if the probe was used in a protein rich
solution the tip should first be soaked in a protease solution for several minutes
to remove protein build-up, and then in distilled water. enzymatic detergent
(
e.g.
, enzol, WPi # 7363 ) can also be used. the membrane sleeves can also be
sterilized chemically using an appropriate disinfectant (
e.g.
, cidex, WPi # 7364 ).
Accumulated organic matter can be removed by briefly immersing the tip in an
acid or base solution (at times both may be necessary) for 0 seconds. A good
indication of a dirty membrane sleeve is a sluggish response or an unusually low
sensitivity. if these problems are not rectified by the above cleaning procedures
then the membrane sleeve should be replaced. the probe cannot be used in
organic solvents.