WPI APOLLO 4000, Инструкция по эксплуатации

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APOLLO 4000
NITRIC OXIDE DETECTION
WORLD PRECISION INSTRUMENTS C-6
Once the sensitivity of the
probe has been ascertained
(in the above example the
sensitivity was 1.557 pA / nM)
the sensor is ready to use
experimentally.
NO
2
and NO
3
Determination
We have recently developed a new reagent-less nitrate-reductor, called the
NITRALYZER™ . The Nitralyzer is a very useful tool for researchers currently using
enzymatic conversion of NO
3
to NO
2
. Researchers who use the Greiss method for
NO
2
determination will also be very interested to learn that using the ISO-NOP (and
the titration method for calibration described above) a detection limit for NO
2
as low
of 1 nM is routinely possible. This compares to a detection limit using Greiss
reagent of approximately 1
µ
M. Hence, the ISO-NOP is several orders of
magnitude more sensitive than the Greiss method. Information on the Nitralyzer is
aavailable from WPI.
Figure C3 —
APOLLO4000
Calibration Curve
ISO-NO Calibration Curve Based on Tabulated Data
0
400
800
1200
1600
0 200 400 600 800 1000
Concentration of NO (nM)