Instruction Manual
K SERIES Cryostorage System
IM: CryoCeK
Revision: 02
Approved by: CB
Page
24
of
87
6 OPERATION OF DEVICE
6.1 SELECTING CRYOPROTECTANTS
The Worthington Industries K SERIES Cryostorage System is designed to operate with liquid nitrogen
at extremely low temperatures from -100 °C to -196 °C under normal atmospheric pressure.
The cryoprotectant selected for long-term storage of cells can have
profound implications for future experimental results:
Different cell types have different permeability for different types of cryoprotective
molecules. This difference affects the rate at which a cell shrinks and swells before
freezing, during freezing, and during thaw cycles.
This can have a dramatic impact on cell viability.
Thus, you need to pay close consideration to the type and concentration of the
cryoprotectant being used. A careful analysis of literature regarding standard operating
procedures for your cell type and small scale experimentation can save the frustration
and cost of having to repeat lengthy experimentation as a result of poor
cryopreservation conditions.
Worthington Industries and the distributors do not assume any
responsibility for the selection of appropriate cryoprotectants to be used
with your K SERIES Cryostorage System!
Due to the large number of influencing factors, this remains to the sole responsibility of
the professional users operating the K SERIES Cryostorage System.
6.2 INITIAL FILL
IMPORTANT
The following safety precautions apply to the Worthington Industries K SERIES
Cryostorage System:
Inlet pressure should not exceed 1.5 bar/152 kPa!
Higher liquid nitrogen supply pressure at the inlet to the refrigerator opens
the relief valve on the refrigerator.
