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vwr.com I Instruction manual VWR

incubator

General safety instructions on installing and operating the CO

incubator

1.2.

Indications for using pressure cylinders

1.2.1.



During the operation of the CO

incubator, small amounts of CO

enters the room, which in high

concentration can be life-threatening. The unit does not emit CO

when the power supply is

switched off and the gas cylinder valves are closed.



The CO

incubator can’t be operated near concentrated acids or corrosive vapours.



The workplace must be properly ventilated.



Emergency shutdown of the CO

dosing: close the gas cylinder valve.

The amount of dispensed CO

is regulated by a microprocessor controller. If the chamber door is open during CO

dosing,

the gas will be automatically cut off.



Together with a pressure cylinder with compressed gas, the user should be given a safety data sheet for the gas and
instructions for its use.



The ventilation performance in the room must ensure level of gas concentrations in the room below the limit values.
High concentration of CO

(>4 Vol.-%)- risk of death by suffocation.



During gas intake from the gas cylinder, check the pressure level regularly.

Characteristics of carbon dioxide

1.2.2.

Carbon dioxide (CO

) is colorless and odorless gas, therefore it is practically imperceptible. CO

is heavier than air and soluble

in water.
Warning signs on the bottle containing CO

Non-flammable and non-toxic gases

Pressurised gas

Product ID: Carbon dioxide (100%)
Chemical formula: CO

Application of the substance: General industrial applications

Содержание INCU-Line Premium

Страница 1: ...Instruction manual VWR INCU Line Premium CO2 incubator EU cat no 390 1544 VWR ILCO 180 Premium ___ Version 01 Issue 07 2021 ...

Страница 2: ...s of Manufacturer Europe VWR International bv Researchpark Haasrode 2020 Geldenaaksebaan 464 B 3001 Leuven 3216385011 be vwr com UK Importer VWR International Ltd Hunter Boulevard Magna Park Lutterworth Leicestershire LE17 4XN uk vwr com Country of Origin POLAND ...

Страница 3: ...lation 11 3 2 Water pan 12 3 3 Placement of samples 13 3 4 Closing the chamber door 13 3 5 Internal glass door 13 3 6 Connection of the CO2 gas cylinder 14 3 7 4 Intended use 15 5 Symbols 15 6 Overview 16 7 Main Switch 17 8 Main screen 17 Alarm bar 18 8 1 Alarms panel 18 8 2 Information panel 19 8 3 Status description 20 8 4 Status protection and alarms 20 8 5 9 Getting started 21 Internal memory ...

Страница 4: ...rections 46 10 14 Hot air sterilization program 47 10 15 11 Components of the CO2 incubator 51 Door lock 51 11 1 Access ports 51 11 2 Open door alarm 52 11 3 USB port 52 11 4 LAN port 53 11 5 12 Temperature protection 53 13 Remote control of the unit 53 14 Troubleshooting 53 15 Service of the unit 54 Replacing the CO2 gas filter 54 15 1 16 Accessories and spare parts 55 17 Cleaning and maintenance...

Страница 5: ...5 vwr com I Instruction manual VWR CO2 incubator 23 Compliance with local laws and regulations 59 24 Equipment disposal 60 Local VWR offices in Europe and Asia Pacific 61 ...

Страница 6: ...chnical data 3 You should ensure sufficient ventilation place samples in such a way to provide proper air circulation in the chamber open the door for the shortest period of time to reduce temperature fluctuations always check that the doors are closed use only earthed mains to avoid electric shocks unplug the power cable holding the protective cover and not the cable itself disconnect the unit fr...

Страница 7: ...troller If the chamber door is open during CO2 dosing the gas will be automatically cut off Together with a pressure cylinder with compressed gas the user should be given a safety data sheet for the gas and instructions for its use The ventilation performance in the room must ensure level of gas concentrations in the room below the limit values High concentration of CO2 4 Vol risk of death by suff...

Страница 8: ...rotection first aid 1 2 5 In the event of gas leak Leave the room immediately warn other people and ventilate the room if possible Respiratory protection In an atmosphere where there is a lack of oxygen excess of carbon dioxide use a self contained breathing apparatus or a face mask with a positive pressure air supply Respirators with air filters will not provide protection Inhalation problems Mov...

Страница 9: ...ual and Premium Control Software can be downloaded from the unit s internal memory on the USB stick Additional shelves a stacking adaptor a base on castors a CO2 gas filter can be ordered separately The INCU Line ILCO 180 is stackable Two units can be stacked on top of each other with the stacking adaptor saving valuable space in the laboratory The stacking adaptor prevents heat transmission shock...

Страница 10: ...all Height of the room must be at least 300 mm greater than the height of the unit Keep away from direct sunlight Kept away from any heat sources Not designed to be built in e g within wall recesses Placed near a mains socket 220 240V 50 60Hz When after storage in a cold location you transfer the chamber to its warmer installation site condensation may form Before start up wait at least one hour u...

Страница 11: ... assemble the rack for shelves water pan and adjust shelf height 1 Install the part a of the rack as close as possible the right wall of the chamber 2 Install the part b of the rack as close as possible the left wall of the chamber 3 Connect the part a with the connecting rod c Note the pin of the rod must be directed towards the door 4 Connect the part b with the connecting rod c Note to lock the...

Страница 12: ...ean the chamber Water pan 3 3 Please make sure that the water pan is placed on the chamber s bottom equally between the rack Below the water pan there is the cold spot of the chamber to avoid water condensation Fill the water pan only with deionized demineralized water up to the filling level marked by the pressed inner edge volume 1 5 liter It is recommended to clean and refill the pan 2 3 times ...

Страница 13: ...space the samples should be placed so that the space between the containers does not exceed 1 3 of the width and height of the shelf samples should be placed with equal spacing between each other and the chamber walls The picture below is an example of the placement of samples in the chamber Figure 1 Samples placement in the chamber Hmax H Hmax a u v w x y z b u v w 1 3 a x y z 1 3 b Hmax 2 3H u v...

Страница 14: ...Leak test After connecting the gas cylinder check all gas connections for leaks e g with leak spray or dilluted soap solution The gas outlet pressure MUST NOT exceed the indicated value of 2 5 bar The pressure above will result in chamber damage Before connecting check the outlet pressure on the pressure reducer of the cylinder Adjust the outlet pressure to 1 5 2 0 bar above the ambient pressure O...

Страница 15: ...y only be loaded with materials and substances which cannot explode burst or ignite do not form any toxic or explosive vapours at the set temperature Potentially explosive gas air mixtures must not form neither in the working chamber nor in the direct vicinity of the appliance The incubator should not be used to sterilize samples The sterilisation programme in the menu will only sterilize the inne...

Страница 16: ...lves 7 CO2 infrared sensor 8 CO2 gas mixing head 9 Temperature sensor PT 100 10 Stainless steel perforated shelves 11 Open door sensor 12 Height adjustable feet 13 Water pan 14 Inner glass door 15 Glass door handle 16 Inspection cover for CO2 sensor and filter 17 Main power switch 18 CO2 hose connector 19 10A fuse 20 Main power socket 21 LAN 19 21 20 16 17 18 7 8 9 6 7 13 14 10 8 11 15 12 4 5 2 3 ...

Страница 17: ...ties the user should log in Main Menu Start Stop the program Current temperature Current CO2 concentration Set temperature to change press the icon or value for more information see 10 3 1 Quick change the set temperature Elapsed or remaining time of program to change press the icon or value for more information see 10 3 2 Quick change the set time Set CO2 to change press the icon or value for mor...

Страница 18: ...Alarms panel 8 2 In this place appears the list of alarms With active alarm the control bar is red The alarm event is displayed in the list with the status alarm active When an alarm event stops the alarm remains in the list as inactive delete button confirms and removes the alarm from the list only inactive alarms can be deleted confirm button confirms the class 3 1 alarm details button displays ...

Страница 19: ...window is active indicates Closed door open door The number above the icon presents open door counter press the icon to cancel the counter The counter is also cancelled during turning on the unit Ramp status Chamber is currently cooling down or heating up to reach set temperature Set temperature is reached Icon is only visible when the chamber is heating Figure 4 Alarm details Figure 5 Status icon...

Страница 20: ... temperature program off Time set Set time of performing segment Time elapsed Elapsed time after reaching the segment Time remaining Remaining time until the end of the segment Status protection and alarms 8 5 In the left part there is information about the alarm class and the upper set value It is associated with a running or completed program The protection parameters can be set in the program p...

Страница 21: ...ay proposing to save the Download folder folder includes Premium Control Software and instruction manual on a USB stick Insert the USB stick not included and wait a second to detect the hardware then press By pressing you quit downloading the folder the window will appear again during the next start You can tick Don t show again so that the window will not be displayed when you start You can alway...

Страница 22: ... the unit If no user is logged in press in the main screen The login window will appear Figure 10 Factory default login parameters login admin leave the password field empty Super Administrator can create max 4 additional users More information 10 8 Users It is advised to set the password during the first run and make a note in a safe place in order to avoid interference of unauthorized people Inf...

Страница 23: ...rom the USB flash drive Information about the number of created programs and the programs to be created is presented at the top Program creating edition 10 1 1 Press the button or and a panel with program parameters will appear Figure 11 Figure 12 You can set the panel Name after pressing the keypad will appear and you will be able to enter the name of the program Segments number the number of seg...

Страница 24: ...f time or parameters more information 10 1 5 Priority Loop the number of program repetitions more information 10 1 4 Loop With more With more parameters you can scroll the panel up and down Cancels adding or editing of the program Switching to the edition of program segments Figure 11 Program parameters 1 ...

Страница 25: ...lect continuous work in the last segment CO2 CO2 concentration to be achieved in the segment ramp time the time of reaching the set temperature d hh mm days hours minutes The active value is highlighted in blue The item highlighted in red means that the value is out of range and you should enter another one eg the temperature is above below the operating range of the unit or the protection tempera...

Страница 26: ... again from the first segment You can define if a program should be carried out once loop 1 or multiple times loop 2 to 255 You can also set the program to be carried out continuously tick the option Priority 10 1 5 Can be set in terms of Parameters The program without a ramp the unit starts the countdown of the segment time when the set temperature is reached The program with a ramp first the uni...

Страница 27: ...he cartridge protection is to protect against uncontrolled rise in temperature At the moment of activation the relay disconnects the heating power supply Temperature protection class 3 1 when the temperature drops below the set protection value the power turns on automatically and after rising the temperature above the set protection value the power turns off automatically The temperature set in t...

Страница 28: ...panel Figure 19 press the icon in the upper right corner Select the program you want to activate Figure 20 you have got two additional options Immediate start of the program Scheduled program start according to the set date and time Figure 17 Main menu Figure 18 List of programs ...

Страница 29: ... the bottom panel Figure 6 Quick change the set temperature 10 3 1 In order to quickly change the value of the set temperature of the program press the icon in main screen Figure 2 The value of the temperature should be selected by scrolling the list up or down Figure 21 Confirm the change by pressing Temperature cannot be higher than protection temperature over temperature 2 C Figure 19 Main scre...

Страница 30: ...t the number of days hours and minutes by scrolling the list up or down Figure 22 Confirm the change by pressing To set the continuous work press To change the way of displaying the time press to display the elapsed time to display the remaining time To change only the way of displaying you do not have to confirm it by Figure 21 Quick change of temperature Figure 22 Quick change of time ...

Страница 31: ...he lowest recorded temperature Maximum temperature C the highest recorded temperature Average temperature C average temperature Minimum CO2 the lowest recorded CO2 concentration Maximum CO2 the highest recorded CO2 concentration Average CO2 average CO2 Segment status of the segment in progress currently performed segment data is constantly updated finished the segment is made interrupted the segme...

Страница 32: ...he latest record It is possible to segregate data according to the selected column To do so click the column heading Data are only registered when the program is running The frequency of registration depends on the program parameters settings The time of opening the Data Register depends on the number of saved data The larger number of stored cells increases the opening time of the window Then a p...

Страница 33: ...gram display see 10 5 1 Graph Deleting data Users with Super Admin rights can delete all data also those registered by other users Figure 27 Graph 10 5 1 It is possible to generate a graph from the available samples in the data register Choose the graph to enlarge by double click Figure 28 Displays the entire graph back to the list of graphs Back to Data Register Figure 26 Data Register Figure 27 ...

Страница 34: ...34 vwr com I Instruction manual VWR CO2 incubator Figure 28 Graph selection Figure 29 Temperature graph Figure 30 CO2 graph ...

Страница 35: ...th right and down at the same time Figure 31 By swiping to the left you can back to the normal size of the graph Event log 10 6 The window displays information about registered events alarms and errors Data is only available for Super Admin users Saving the data onto USB stick csv files are available semicolon separated by opening e g with a spreadsheet plkx opening with the Premium Control applic...

Страница 36: ...eted Measurement user measurements have been deleted Deleted All Measurement all measurements have been deleted User added new user has been added User updated user has been changed User deleted user has been deleted Program saved new program has been saved Program deleted program has been deleted Program updated program has been updated Lower CO2 alarm Start lower CO2 concentration alarm has been...

Страница 37: ...he suggestion to save the unit configuration file on the USB stick appears Figure 34 The file is used to create an offline program in the Premium Control application Users 10 8 In this panel Figure 35 the Super Administrator can add a new user edit an existing one or delete it Max 5 different users can be created and max 40 programs can be freely distributed among these 5 users Add a new user Edit...

Страница 38: ...data will appear Figure 36 Only the Super Administrator can create and edit users Should be given login a user name password account password password confirmation enter the password again to confirm it type type of account Super Admin and Admin users have only access to type account Admin More information in chapter 10 8 2 Types of accounts and their limits programs limit the number of programs t...

Страница 39: ...programs check their statistics statistics and data register and information about the system info Super Admin account Super Admin account has not limits It has access to the program menu management and to the settings menu Confirms and saves the user Cancels the entered data and returns to the list of users The unit can have up to 5 users incl Super Administrator Super Administrator can distribut...

Страница 40: ... In this panel Figure 41 you can View available language of the controller Set the time after which the screen will be dimmed Turn on off the sound alarm sounds will be still emitted Set the time after which the user will be automatically logged out Figure 39 Main menu Super Admin 2 Figure 40 Main menu Super Admin 3 ...

Страница 41: ... If you change the date and time to an earlier than the current one a message will appear on the display Changing the date time will interfere with the records in the database Delete the data from data record and event log first then change the date time You must first delete the data from the data record and from event log and then change the date time Confirms and saves changes Cancels the enter...

Страница 42: ... drops below the value given in this field upper alarm CO2 an alarm will be generated if the CO2 concentration rises over the value given in this field The lower and upper alarm can only be generated after reaching the set temperature delay exceed alarm an alarm will be activated with a delay 1 min 2 min 5 min 15 min after exceeding the permitted temperature CO2 concentration delay door alarm a do...

Страница 43: ...or router s that manages the Ethernet network DNS IP address of the domain name system MAC the address of the network card read only DHCP you can select if the server that allocates IP addresses is running on the local network You can then skip setting IP Masks Gates Icon indicates the connection status Incubator connected Incubator disconnected Confirms and saves changes Cancels the entered chang...

Страница 44: ... needed to activate notifications via e mail The panel is divided into three windows Selection of event types for which notifications should be sent Activate activate or deactivate e mail notifications Door alerts alarms caused by the half open door Critical alerts critical alerts e g sensors damage Temperature alerts alarms caused by too high or too low temperature CO2 alerts alarms caused by too...

Страница 45: ...he entered changes If you do not enable the activate option at the top of the panel emails will not be sent Sender and recipients Figure 47 Sender sender s e mail address Recipients recipient s e mail addresses up to 3 Confirms and saves changes Cancels the entered changes Figure 47 E mail sender and recipients ...

Страница 46: ...et correct temperature value indicated on the display by adding the correction value The set correction value is taken in the whole temperature range operation of the unit For example if the average temperature displayed by the unit indicates 37 C and the average temperature measured by independent external sensor indicates 37 5 C the correction should be set on 0 5 C The average temperature shoul...

Страница 47: ... changing of manufacturer s settings If the equipment was calibrated calibration certificate loses its validity Before starting hot air sterilization the entire interior must be clean and dry No residue of e g samples plastic or water can remain inside the chamber empty the water pan the rack shelves water pan and CO2 sensor can stay inside the chamber close the inner glass door and the outer cham...

Страница 48: ...om the chamber Confirm the message The program starts immediately or when the program delay time is over The program will take approx 14 hours and consists of the following steps 1 Heating up phase the inner chamber is heated up as fast as possible to the sterilization setpoint temperature 2 Holding phase setpoint temperature is kept for 2 hours The sterilization setpoint temperature is set to 180...

Страница 49: ...2 sensor can be hot air sterilized inside the chamber The effective sterilization process heating up and holding is automatically finished after holding 180o C inside the chamber for 2 hours The information of successfully perfomed process pops up on the controller display Figure 53 Figure 53 Successful sterilization The total cycle of sterilization finishes when the temperture inside the chamber ...

Страница 50: ... performed sterilization pops up on the display Figure 55 and the event is registered in the Event log Sterilization failed If the sterilization cycle was terminated prematurely the germs inside the chamber might not be killed totally It is recommended to repeat the sterilization Figure 55 Sterilization failed If the door is open when the sterilization program is starting the program won t start T...

Страница 51: ...n be used to insert external sensors which have been secured with silicone plugs The plugs should cover the access ports while the unit is operating If multiple cables have been inserted through the access port and if it is not possible to use the plug secure the access port with adhesive tape If you leave the access port open it may affect temperature variation and fluctuation within the chamber ...

Страница 52: ...e list with the status alarm active USB port 11 4 To transfer data from the unit s memory to the USB memory stick connect the memory stick to the USB slot on the unit s front Go to Setup Data record press the button Select file type csv plkx Press Data is copied Data saved as csv file can be opened in the Notepad Data saved as plkx file can be opened only by Premium Control This program allows you...

Страница 53: ... 3 1 according to DIN 12880 norm is called a protecting sample function The user can set the temperature protection upper limit by himself and when the set temperature falls outside of this range the power heater is switched off When the temperature returns to within normal parameters the equipment continues to work normally Remote control of the unit 13 Every Premium unit can be connected to the ...

Страница 54: ...if the CO2 gas filter is not clogged Replace the filter according to 15 1 Replacing the CO2 gas filter Check if the CO2 gas filter was replaced according to schedule Check the CO2 pressure on the pressure regulator approx 1 5 2 bar Adjust the pressure regulator for proper CO2 pressure approx 1 5 2 bar Service of the unit 15 Replacing the CO2 gas filter 15 1 When the chamber is in operation the inc...

Страница 55: ...ry conditions they do not rust However it is possible that stains which may look like rust form on the steel surface e g due to the kind of samples that are incubated in the chamber In such case it is recommended using cleaning solution to clean the stains which is dedicated to this particular application e g Pelox Exterior cleaning 17 1 1 The housing and door should be cleaned with caution using ...

Страница 56: ... to safely store the unit 18 1 Close the CO2 pressure cylinder valve and disconnect the CO2 gas hose 2 Remove load from the chamber 3 Clean the chamber and empty the water pan 4 Before disconnecting the unit from the mains the chamber must be dried e g by door opening chamber ventilating or chamber heating without the water pan Turning the unit off without drying will cause water condensation in C...

Страница 57: ... product identification label is located on the left upper corner of the unit An example of the product label is shown on the figure below 1 Logo 2 Type of the unit 3 Catalogue number 4 CE mark 5 Name and address of the manufacturer 6 Serial number 7 Bar code 8 UKCA mark 7 6 1 5 4 3 2 8 ...

Страница 58: ...essor with a large 7 full colour touch screen Interior stainless steel to DIN 1 4301 Housing powder coated sheet Overall dims2 mm A width 700 B height 920 C depth 780 Internal dims mm D width 560 E height 650 F depth 500 Max shelf workload kg 10 Max unit workload kg 30 Nominal power W 1700 Weight kg 96 Temperature fluctuation3 at 37 C C 0 1 Temperature variation4 at 37 C C 0 3 Recovery time after ...

Страница 59: ...uals and any local regulations such warranty turns invalid except to the extent the defect of the product is not due to such non performance Items being returned must be insured by the customer against possible damage or loss This warranty shall be limited to the aforementioned remedies IT IS EXPRESSLY AGREED THAT THIS WARRANTY WILL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND IN LIEU OF THE WARRAN...

Страница 60: ...nd recycling It s also your responsibility to decontaminate the equipment in case of biological chemical and or radiological contamination so as to protect from health hazards the persons involved in the disposal and recycling of the equipment For more information about where you can drop off your waste of equipment please contact your local dealer from whom you originally purchased this equipment...

Страница 61: ...WR International Ltd VWR International Northern Ireland Ltd Orion Business Campus Northwest Business Park Ballycoolin Dublin 15 Tel 353 01 88 22 222 Email sales ie vwr com Italy VWR International S r l Via San Giusto 85 20153 Milano MI Tel 39 02 3320311 02 487791 Email info it vwr com Korea VWR International 17 Daehak 4 ro Yeongtong gu Suwon si Gyeonggi do Tel 82 31 645 7256 saleskorea avantorscie...

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