16 – VWR STandaRd Line PiPeTTORS
instruction Manual – 17
Temperature
(°C)
Pressure (kPa)
95.0
101.3
105.0
20
1.0028
1.0029
1.0029
21
1.0030
1.0031
1.0031
22
1.0032
1.0033
1.0033
23
1.0034
1.0035
1.0036
24
1.0037
1.0038
1.0038
25
1.0039
1.0040
1.0040
See iSO 8655 for the full table of conversion factors (Z).
NOTE:
Pipetting should be performed in accordance
with guidance described in Sections 7 and 8.
Checking the Pipetting Accuracy Parameters
•
Set the preset volume depending on the pipettor vol-
ume according to the following table.
Nominal
volume
(μL)
Preset
volume
(μL)
Permissible
values (μL)
Volume Change ΔV (μL)
for a Turn of a Calibration
Key by
1
Turn
1
Increment
Single-channel Pipettors
2
0.2
0.176 - 0.224
0.06
0.0025
10
1
0.975 - 1.025
0.33
0.0137
20
2
1.94 - 2.06
0.63
0.0262
100
10
9.84 - 10.16
2.50
0.104
200
20
19.76 - 20.24
6.30
0.262
1,000
100
98.4 - 101.6
25.00
1.04
5,000
500
494 - 506
125.00
5.2
10,000
1,000
975 - 1,025
250.00
10.4
8-channel and 12-channel Pipettors
10
1
0.92 - 1.08
0.33
0.0137
50
5
4.8 - 5.2
1.67
0.070
200
20
19.4 - 20.6
6.30
0.262
300
50
49.2 - 50.8
10.00
0.42
•
Perform 10 aspirations, and calculate the average
value in [mg].
•
Calculate the volume in [μL] by multiplying the value
in [mg] by the conversion factor Z [μL/mg].
if the average aspirated volume exceeds the permissible
values of the range, the pipettor should be recalibrated.
Pipettor Recalibration
K
1.
2.
3.
4.
1
. Remove the pipetting pushbutton.
CAUTION
: The pipetting pushbutton consists of
2 parts: the knob and the pushbutton. after removal
of the pushbutton, both parts are separated.
2
. insert the calibration key into the calibration screw.
3
. Holding the volume setting knob to prevent rotation,
turn the key clockwise to reduce the aspirated vol-
ume, or counter-clockwise to increase the aspirated
volume. Use the values given in the above table to
precisely adjust the volume.
4
. Remove the key and replace the pipetting pushbutton
by first replacing the knob, then the pushbutton.
determine the average aspirated volume. The average
volume should be within the permissible range given in
the table. if the volume exceeds the values stated, the
recalibration procedure should be repeated.
When pipetting liquids with physical properties consid-
erably different from those of water, follow the guidance
given in Section 5.
More information on the calibration procedure can be
found at
www.vwr.com
