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Measure Concentration by Using The Linear Regression Curve
The following procedure shows how to measure concentration of samples.

1. Set up linear regression curve or click

to open a file of linear regression curve

(*.QUA).

2. Place reference into the sample holder. Click

to do blank.

3.  Click the Sample tab.
4.  Place Sample 1 into the sample holder.
5.  Click Start to run a measurement.
6.  UV-Vis  Application  Software  will  display  the  photometric  value  of  Sample  1  at  the

fixed wavelength positions automatically. Type the sample name in the Name box.
The default is Sample-1.

7. Click OK. The photometric result for Sample-1 will be listed in the sample data.

Delta Abs. and concentration value of Sample-1 will also be displayed in columns 3
and 4.

8. Repeat steps 4-7 to measure remaining samples.

Содержание 634-6002

Страница 1: ...VWR Collection Manual ver 1 rel 15 05 2007 UV 3100PC Spectrophotometer INSTRUCTION MANUAL European Catalogue Number s 634 6002 Version 1 0 0 Issued 07 April 2010...

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Страница 3: ...Legal Address of Manufacturer Europe VWR International Europe bvba Researchpark Haasrode 2020 Geldenaaksebaan 464 B 3001 Leuven Tel 32 16 385 011 http www be vwr com Made in China...

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Страница 5: ...ons and Switches 4 Operational Keys 6 Getting Started 8 Important Guidelines 9 General Operating Instructions 10 Operation 11 Basic Mode 12 Quantitative Mode 14 Wavelength Scan 19 Kinetics 21 DNA Prot...

Страница 6: ...rocessing Function 46 System Check and Calibration Function 46 Installation 47 Introduction 50 Operation 55 Single Wavelength Photometric Measurement 55 Fixed Point Measurement 55 Wavelength Scanning...

Страница 7: ...Part 1 Spectrophotometer...

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Страница 9: ...n any way damaged or defective Repairs may only be carried out by the service technicians from your local VWR office and authorized contractual partners The device must be connected to a power outlet...

Страница 10: ...Check to confirm instrument power switch is turned off connect the printer s data cable to the Instrument s parallel port Link the power cord Check to confirm instrument power switch is turned off th...

Страница 11: ...fications Optical System Single Beam Wavelength Range 190 1100nm Band Width 2nm Stray Light 0 05 T 220nm 360nm Photometric Range 0 200 T 0 3 3 0A 0 9999C Wavelength Accuracy 0 5nm Photometric Accuracy...

Страница 12: ...asy to use simple structure and pretty figure Besides these the large LCD High Precise A D and easy to store RAM makes the instrument much more superior than other originals It is widely used in Chemi...

Страница 13: ...5 Rear View...

Страница 14: ...6 Operational Keys Key Functions...

Страница 15: ...CLEAR Key Delete the input value or stored data ESC Key Return to previous Interface 100 T 0Abs Key Blank OPEN Key Open files stored in internal memory SAVE Key Save files to internal memory START STO...

Страница 16: ...g chart describes the basic operation of the instrument Turn On and Self check Switch on the power Then the instrument begins to self check and 15 minutes warm up Self check includes the following ste...

Страница 17: ...Dn Select WL 500 0nm 10 00 00 Main Menu Basic Mode Quantitative Mode Wavelength Scan Kinetics Mode DNA Protein Mode Multi Wavelength Mode System Utility Important Guidelines Reagents and dilution buff...

Страница 18: ...o to the point you set then do blank automatically Set Parameters In different application press function key to set parameters press to choose or input the values by numeric keys press to enter into...

Страница 19: ...e name by numeric keys and press to save Load the Stored File In the test interface press to go into file selecting interface press to choose the file you want press to open Operation Self check Remov...

Страница 20: ...choose Basic Mode then press to go into 2 Set Photometric Mode Press to set photometric mode Press to choose Abs T or Conc Factor and press to confirm If users choose Abs or T please go to step 5 dire...

Страница 21: ...t the mark Input the concentration value of the standard and press to confirm then it displays on the screen 5 Set Wavelength Press to set wavelength input the value by the numeric keypad followed wit...

Страница 22: ...national bvba Quantitative Mode 1 Enter into Quantitative Mode Main menu press or press to choose Quantitative Mode followed with pressed to confirm 2 Set Unit Press to set concentration unit press to...

Страница 23: ...et Wavelength Press to set wavelength Use to choose measure method then press to confirm Input the wavelength value you need and press to confirm 3 Input the Factor of the Regression Equation Press an...

Страница 24: ...les Press to setup standard input the concentrations of corresponding standard samples according the indication and press to confirm Users can use to choose the value you just input and press to delet...

Страница 25: ...into files select interface Use to select the curve you need and press to load Users can press to view the curve press to cancel 0 Conc 4 4 Return the sample measurement interface In the Calibration...

Страница 26: ...n the data sheet Repeat this step to finish measuring all the samples 7 Print the Test Results Press to print the Test Results QuantitativeTest Report FileName Abc Date Time mm dd yyyy hh mm ss No 500...

Страница 27: ...s to set parameters set Scan From scan to scan step and scan speed press to confirm 3 Set Photometric Mode Press to set photometric mode choose T Abs or E and press to confirm 4 Scan Baseline Put Refe...

Страница 28: ...ut the peak height and press to confirm Press to display the value of every wavelength point Press to display the value of every peak 0 000 Abs 1 200 0 000 Abs 1 200 7 Smooth the Curve After scanned i...

Страница 29: ...0nm Scan From 230 0nm Scan Step 1 0nm Peak Height 0 030Abs Model UV 3100PC SN UEFXXXXXXX Version A1 176 VWR International bvba Peak list No Wavelength nm Abs T 1 536 0 0 918 12 1 0 Abs 1 200 Kinetics...

Страница 30: ...onfirm 3 Set Photometric Mode Press to set photometric mode choose T or Abs and press to confirm 4 Set Wavelength Press to set wavelength input the value of the wavelength by numeric keypad and press...

Страница 31: ...Begin Time End Time and Factor separately and press to confirm Then the value of I U would display on the screen D2 W WL 500 0nm 10 00 00 0 Time s 180 Total T 180s Interval 1 0s I U 0 001 99 5 T 100 5...

Страница 32: ...dd yyyy hh mm ss Wavelength 500 0nm Total Time 180s TimeInterval 1 0s Factor 1 000 Model UV 3100PC SN UEFXXXXXXX Version A1 176 VWR International bvba 0 005 Abs 0 005 DNA Protein Mode 1 Enter into DNA...

Страница 33: ...r Absorbance Difference 2 followed with pressed to confirm If users don t want to measure reference use to choose No followed with pressed to confirm the choice 4 Set Concentration Unit Press to set c...

Страница 34: ...d yyyy hh mm ss Sample 1 260 0nm 0 251Abs 280 0nm 0 243Abs 320 0nm 0 095Abs C DNA 4 524ug mL C Pro 110 8ug mL Ratio 1 059 Unit ug mL Model UV 3100PC SN UEFXXXXXXX Version A1 176 VWR International bvba...

Страница 35: ...by one by the numeric keypad Press to confirm press to return 3 Set Photometric Mode Press to set the photometric mode use to select Abs or T mode press to confirm 4 Blank Place the Reference Sample...

Страница 36: ...velength Test Report FileName Abc Date Time mm dd yyyy hh mm ss Sample 1 500 0nm 0 251Abs 510 0nm 0 243Abs Unit Abs Model UV 3100PC SN UEFXXXXXXX Version A1 176 VWR International bvba System Utility M...

Страница 37: ...tment is prohibited Printer Setup Press or to select Printer Setup then press to confirm Reset Printer Press or use to choose Reset Printer and press to confirm Then the printer will resume the initia...

Страница 38: ...ess to confirm Two modes are under your choice one is Print Data Sheet the other is print the display interface Lamp Service Press or use to choose Lamp Service press to go into lamps management inter...

Страница 39: ...are sure to reset the usage time Reset the W lamp usage time Press or use to choose Reset W lamp usage time and press Then users can find the W lamp s usage time and the system will ask if you are su...

Страница 40: ...e Set Date and press to confirm Input the Date Year Month Day by numeric keypad press to confirm and return automatically Display Time Press or use to choose Display Time and press to confirm Then the...

Страница 41: ...ting interface When the instrument was connected to PC it displays Controlled by PC Beeper on off Press or use to choose Beeper on off and press to switch on or switch off the beeper Language Selectio...

Страница 42: ...ngs Use to choose Restore Default Settings and press to Confirm then the system will restore the initialization Troubleshooting Review the information in the table below to troubleshoot operating prob...

Страница 43: ...k current error Resample dark current Repair and Maintenance Daily Maintain Check the compartment After measurement the cuvettes with sample solutions should be taken out of the compartment in time Or...

Страница 44: ...unplug the socket before replacement 1 Tools preparation Prepare a 3 75 Flat Blade screwdriver 2 Switch Off the power supply Switch off the power supply and unplug the socket 3 Take out the Fuse Seat...

Страница 45: ...amp chamber after power off to avoid scald 1 Tools preparation Prepare a 6 150mm Cross Blade screwdriver and a pair of glove 2 Power Off Switch off the power supply and unplug the socket 3 Open the co...

Страница 46: ...remove the D2 lamp Draw on the cotton glove and replace a new lamp Fix the 2 screws and plug the connector again 6 Replace W lamp Pull out the defected W lamp and draw on the cotton glove Insert the n...

Страница 47: ...f the entrance hole If the facular deviate to Left or Right then loosen the No 1 screws in Fig 5 8 and move the lamp seat to Left or Right until it focus on the center of the slot Then fix the screws...

Страница 48: ...he course finished Replace the Battery Be sure to switch off the power supply and unplug the socket before open the Bottom Cover 1 Prepare the tools Prepare a 6 150mm Cross Blade Screwdriver 2 Switch...

Страница 49: ...l the screws then the course finishes Accessories and Spare Parts Description Quantity Cat No CELL HOLDER 4 CELL 10MM 1PC 634 6003 CELL HOLDER 4 CELL 10 TO 50MM 1PC 634 6004 CELL HOLDER 4 CELL 10 TO 1...

Страница 50: ...2PCS 634 6020 CUVETTE SQUARE QUARTZ 50MM 2PCS 634 6021 CUVETTE SQUARE QUARTZ 100MM 1PC 634 6022 CELL QUARTZ 100UL 10MM 1PC 634 6023 CELL QUARTZ 200UL 10MM 1PC 634 6024 CELL QUARTZ 500UL 10MM 1PC 634...

Страница 51: ...uct will be free from defects in material and workmanship for a period of two 2 years from date of purchase If a defect is present VWR will at its option repair replace or refund the purchase price of...

Страница 52: ...r responsibility to decontaminate the equipment in case of biological chemical and or radiological contamination so as to protect from health hazards the persons involved in the disposal and recycling...

Страница 53: ...Part 2 Software...

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Страница 55: ...sing a linear equation that fits the data Enter factor values to generate regression curve 3 methods for curve fit Linear fit Quadratic fit and Cubic fit Wavelength Scanning Allow user to set scan ste...

Страница 56: ...rivative spectrum is useful for enhancing spectrum data that are not readily apparent in an absorbance spectrum Calculate Spectrum You can calculate addition subtraction multiplication and division be...

Страница 57: ...ory 256MB or Above is strongly recommended 50MB or above hard disc Space Microsoft Windows 2000 XP Vista 7 Install UV Vis Analyst 1 Put UV Vis Analyst disc in the CD ROM 2 Double Click to open the CD...

Страница 58: ...48 4 Choose install path then click Next 5 Select setup type then click Next 6 Select program fold Click Next to copy files to PC...

Страница 59: ...s Analyst There are two ways to start the UV Vis Analyst Double click shortcut icon on the desktop Start All Program UV Vis Analyst UV Vis Analyst Set up Communication Port Start the UV Vis Analyst on...

Страница 60: ...art Menus Bar and Tools Bar Menu bar and Toolbar are both provided in the software offering you two ways to select a desired function On the menu bar use your keypad or mouse to select the desired fun...

Страница 61: ...ent New a Instrument Validity Open Open a spectrum data file Close Close current measurement Save Save current measurement Save As Save current measurement as a Open file from Open a file saved in ins...

Страница 62: ...the area selected Restore Restore the default parameters Search Search peak valley one by one UV Photometer Link Connect to the Instrument Reset Reset parameters of instrument Escape Stop current mea...

Страница 63: ...rt Setup Setup comm port Change Password Set Change login password Auto sample Locate Cell Locate cell 1 8 to light path Setup Multicell Setup Multicell Autorun Measure multi samples Scan Start Start...

Страница 64: ...Derivate Derivative of a spectrum Resample Resample a spectrum Window New Window New a measurement window as Cascade Multi windows display in a cascade Tile Multi windows display in a tile Arrange Ico...

Страница 65: ...hotometric value at a fixed wavelength 1 Click on the toolbar appears Goto specified wavelength 2 Key in the desired wavelength position click Goto The minimum wavelength step is 0 1nm in a range from...

Страница 66: ...mber of WL Points box or click the up down arrows next to the box set the wavelength points Leave the two boxes Calculate Concentration and Use Standard Samples 4 Key in the wavelength in the Waveleng...

Страница 67: ...nt Click Start or to run a new measurement The display will change to the following Key in the sample name in the Name box 8 Click OK The photometric data for sample will be listed in the Sample table...

Страница 68: ...is calculated and its value at the second wavelength is subtracted from the absorbance at the second wavelength to give the peak height 4 Key in the wavelengths in the Wavelength boxes 5 Tick the Cal...

Страница 69: ...gression curve 1 Leave the Use Standard Samples check box 2 Click down arrow in Curve Fit box to select curve fit method 3 Input the factor of the linear regression curve 7 Click Fitting tab to view t...

Страница 70: ...Sample tab 4 Place Sample 1 into the sample holder 5 Click Start to run a measurement 6 UV Vis Application Software will display the photometric value of Sample 1 at the fixed wavelength positions au...

Страница 71: ...ribes how to collect a spectrum while using Wavelength Scan function Scan Sample 1 Click on the toolbar to new a sample scan measurement appears the following form 2 Click on the toolbar appears the f...

Страница 72: ...or 30 click OK 3 Click on the toolbar to select Transmittance mode or click to select Absorbance mode 4 Click on the toolbar to set display parameters 5 Place reference into the sample holder Click t...

Страница 73: ...3 Auto List Peaks and Valleys Click on the toolbar to set the peak Valley threshold range 0 to 1 000 step 0 001 Input the threshold value click OK Click to list peaks and click to list valleys Rescale...

Страница 74: ...he toolbar to activate zoom function Position the cursor in the upper left corner of the area you want to select Hold the left mouse button to drag the cursor to outline the spectrum area you want to...

Страница 75: ...d Y axis values for the current cursor location Double click the left mouse button to release the crosshair cursor Select a Spectrum as Current As UV Vis Application Software can display several spect...

Страница 76: ...er 1st 2nd 10th derivative is required and type a name for the result spectrum then click OK The result spectrum will be displayed overlaid with the original one 4 3 2 1 Moving Window Averaging Click...

Страница 77: ...Computer menu click Savitzky Golay Smoothing Filter Appears following form Click up down arrow to select the parameters key a file name in the Name of Result box click OK The result spectrum will be...

Страница 78: ...ng dialogue box will be displayed Click the down arrow next to File 1 to select a spectrum and define it as source 1 Select a spectrum for File 2 in the same way It will not allow you to select the sa...

Страница 79: ...Click on the toolbar The following dialogue box will be displayed Click the down arrow next to File 1 to select a spectrum and define it as source 1 Select a spectrum for File 2 in the same way It wi...

Страница 80: ...on the toolbar The following dialogue box will be displayed Click the down arrow next to File 1 to select a spectrum and define it as source 1 Select a spectrum for File 2 in the same way It will not...

Страница 81: ...ick on the toolbar The following dialogue box will be displayed Click the down arrow next to File 1 to select a spectrum and define it as source 1 Select a spectrum for File 2 in the same way It will...

Страница 82: ...the display Define Display Information Click on the toolbar appears the Settings to display and print the spectra form click the Legend tab type the information for display Edit Print Information Cli...

Страница 83: ...ill appear 2 Click on the toolbar to select the transmittance mode or click to select the absorbance mode 3 Click on the toolbar A dialogue box will be displayed Key in the wavelength total time in se...

Страница 84: ...ment will start scanning automatically The graph will be displayed on the screen during time scanning You can stop scanning by clicking Calculate Rate Click on the toolbar appears the Settings to disp...

Страница 85: ...mation for display Edit Print Information Click on the toolbar appears the Settings to display and print the spectra form click the Legend tab type the information for display DNA Protein Measurement...

Страница 86: ...Protein Conc 3 Place a reference in the sample holder Click on the toolbar to do blank 4 Click the Sample tab It will display the following The control menu contains six buttons Start Delete Modify Re...

Страница 87: ...c value of sample 1 at the fixed wavelength automatically Key in the sample name in the Name box Click OK after the measurement is complete The photometric data for sample 1 will be listed in the samp...

Страница 88: ...A3 Appendix 2 Methods of DNA Protein Analysis Method 1 CDNA A1 Aref f1 A2 Aref f2 CProtein A2 Aref f3 A1 Aref f4 Ratio A1 Aref A2 Aref A1 A260nm A2 A280nm Aref A320nm Optional f1 62 9 f2 36 0 f3 1552...

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Страница 90: ...nyola del Vall s Barcelona Tel 902 222 897 Fax 902 430 657 E mail info es vwr com Sweden VWR International AB Fagerstagatan 18a 163 94 Stockholm Tel 08 621 34 00 Fax 08 621 34 66 E mail info se vwr co...

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