VWR International 634-6002, Инструкция по эксплуатации

Страница: 68 / 90

58
Concentration Measurement
Set Up Linear Regression Curve
There are two methods available to set up the linear regression curve. You can use
standards to set up the regression curve or just key in the parameters manually. Use the
following steps to select the method you wish to use.
1. Click on the toolbar.
2. Click the Method tab.
3. Enter the number of wavelength points in the Number of Points box, or click the
up/down arrow next to this box. With 2 wavelengths, the absorbance at the second
reference wavelength is subtracted from the first to correct for background
absorbance. With 3 wavelengths, the baseline between the first and third
wavelengths is calculated and its value at the second wavelength is subtracted from
the absorbance at the second wavelength to give the peak height.
4. Key in the wavelengths in the Wavelength boxes.
5. Tick the Calculate Concentration check box to activate concentration calculation.
6. Set up the linear regression curve.
Method 1: Set up the linear regression curve with prepared standards.
(1) Tick the Use Standard Samples check box.
(2) Place the reference into the sample holder. Click to do blank.
(3) Click the Standard tab.
(4) Place Standard 1 in the sample compartment. Click Start to run a
measurement.
(5) Key in the concentration value of Standard 1 in the Conc. box.
(6) Key in the sample name for the standard in the Name box.
(7) Click OK. The photometric data,
△
A and concentration will be shown in the
standard table.
(8) Repeat steps 4-7 to measure all the prepared standards.