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81-0220-01 Rev R
8
Capturing Images and Using Templates
8.1
Image Focusing
8.1.1
Manual Lens
Prior to capturing an image, prepare the image focus:
1.
Turn on the transilluminator and place the
Fluorescent Focus Target
(see “System
Components”) on the transilluminator surface.
NOTE
: The darkroom has a UV safety switch that turns off the transilluminator and op-
tional UV handheld lamps when the door is open. After closing the door, open the
UV
Gel Viewer Window
to ensure that the transilluminator is on and that all other lighting is
off.
2.
With VisionWorks open, preview the image. Adjust the camera settings, including
exposure time, to enhance the image of the sample. To adjust the settings, go to the
Acquisition Action Tab and click on the Camera Menu Button. Adjust the exposure time
from the Exposure Time section of the Camera Menu.
3.
Rotate the lens f-stop adjustment (top ring) so that the image is bright enough to be
seen on the screen.
4.
Rotate the lens focus adjustment (bottom ring) on the lens. Adjust the focus so that the
image appears clear on the screen.
5.
Rotate the zoom lens adjustment (middle ring, if zoom lens is used) on the lens so that
the image is ideally zoomed. Readjust the focus ring (bottom ring) on the lens, making
the image clear. Adjust the zoom so that the object of interest is within the image pre-
view area.
