System design
10
81-0220-01 Rev R
4.2
Camera Models
815 CCD and 515 CCD Cameras
Specifications
815 CCD
515 CCD
Type
CCD, Monochrome
CCD, Monochrome
Resolution
3296 x 2472, 8.1 MP
2.1 MP
Bit Depth
16 bit
16 bit
Quantum Efficiency
50 % peak
50 % peak
Cooling
- 57 °C from ambient
- 57 °C from ambient
Binning
Yes, 1 x 1 through 8 x 8 Yes, 1 x 1 through 8 x 8
PC Interface
USB2.0
USB2.0
4.3
Ethidium Bromide (EtBr) emission filter
The ethidium bromide (50 mm
2
) UV-blocking bandpass interference filter blocks UV and IR
radiation emitted from the transilluminator. The filter is placed in the slide-out filter wheel
assembly on the side of the Imager. The filter allows visualization of fluorophores from 580 -
630 nm, targeting the ethidium bromide emission peak of 605 nm.
Additional filters are available for other specific fluorophores. Filters can also be removed when
imaging non-fluorescent media (including chemiluminescent blots, protein gels, colony plates,
etc.) in order to produce brighter images.
4.4
Transilluminator
The GelDoc-It
2
and ChemiDoc-It
2
Imagers can accommodate UVP’s Benchtop and FirstLight
transilluminator models. UVP offers a variety of transilluminator configurations, including
Benchtop models with multiple wavelengths and variable intensities as well as the highly
uniform, patented FirstLight
®
transilluminator. Refer to the packing slip for the transilluminator
included with your system.
Note:
For UV protection and to extend the life of the UV transilluminator, the system
incorporates a ten-minute transilluminator shutoff timer. The timer shuts off the
transilluminator after ten minutes of operation. A push button on the front of the
darkroom allows the user to reset the timer. Alternately, the timer can also be reset by
opening and closing the darkroom door.
4.5
Fluorescent Focus Target
The Fluorescent Focus Target fluoresces when placed
on a UV transilluminator or when exposed to overhead
UV. The Target provides sharp fluorescent images to
aid in adjusting the lens and camera settings for ideal
imaging results.
