Unico S-1100, Руководство по эксплуатации

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8
Compartment again and close the lid.
7. Record the Absorbance reading on the Digital Display window.
8. Advance the wavelength setting by 1 nm and repeat steps 4 to 7 .
9. Repeat step 8 until the wavelength setting reaches 370 nm.
10. Look for the maximum absorbance reading obtained, and this should be found within
359~363 nm. The wavelength accuracy of the unit is
± 2 nm.
Didymium Filter Method
1. Set the Wavelength to 800 nm.
2. Make sure the cuvette adapter is empty and place it in the Sample Compartment. Close the
lid.
3. Set zero Abs by pressing the 0A/100%T (
∧
) button. Wait a few seconds when the display
flashes. The reading should then be 0.000A. If not, repeat step 3 .
4. Remove the Cuvette Holder and insert the Didymium filter into it. Place it in the Sample
Compartment and close the lid.
5. Record the Absorbance reading on the Digital Display window.
6. Advance the wavelength setting by 1 nm and repeat steps 2 to 5 .
7. Repeat step 6 until the wavelength setting reaches 815 nm.
8. Look for the maximum absorbance reading obtained, it should be within 805-809 nm. The
wavelength accuracy of the unit is
± 2 nm.
9. If a “middle” wavelength check is desired, set the wavelength to 522 nm (optional)
10. Make sure the cuvette adapter is empty and place it in the Sample Compartment. Close the
lid.
11. Set zero Abs by pressing the 0A/100%T (
∧
) button. Wait a few seconds when the display
flashes. The reading should then be 0.000A. If not repeat step 11 .
12. Remove the cuvette adapter and insert the Didymium filter into it. Place it in the Sample
Compartment again and close the lid.
13. Record the absorbance reading on the Digital Display window.
14. Advance the wavelength setting by 1 nm and repeat step 10 to 13 .
15. Repeat step 14 until the wavelength setting reaches 536 nm. Again, look for the maximum
absorbance reading. It should be within 527-531 nm.
Absorbance Accuracy Check
Specification: ± 2% at 1A ( 65-3303 )
The absorbance accuracy should be checked against a set of neutral density filters accurately
calibrated to the NIST standards. Contact your
Carolina representative for more information.
Stray Light Check
Specification: Less than 0.5%T at 340 nm by ASTM (American Society for Testing and Materials)
E 387 (Molecular Spectroscopy and Separation Science Standards), also see
www.astm.org