6
B.
Sample Preparation
1. Make a blank reference solution by filling a clean square cuvette (or test tube)
half full with distilled/de-ionized water or other specified solvent. Wipe the cuvette with tissue
to remove the fingerprints or droplets of liquid.
2. Insert the blank square cuvette into the square cuvette adapter and place the
adapter in the Sample Compartment (if using test tube, insert it directly into the Sample
Compartment, aligning the guide mark (if present) with the guide mark at the front of the
Sample Compartment). Close the lid.
3. Set 0.000A or 100%T with the
0A/100%T
button.
NOTE
: This step fulfills the instructions on the front of the spectrophotometer
4.
Remove the blank square cuvette or test tube. Set it aside in case you may need to
adjust the 0A/100%T using
0A/100%T
button later (i.e. change the wavelength).
C.
Sample Analysis
1.
Rinse a second square cuvette with a small amount of the sample solution to be tested.
Fill the square cuvette half full and wipe clean the exterior of the cuvette.
2. Put the sample cuvette in the Sample Compartment. Close the lid.
3. Read the
T
or
A
from the Digital Display window. Remove the sample cuvette or
test tube.
4. If you are to test the same sample at other wavelengths, repeat
step 3 to B. 1
–
4
for each wavelength.
5. For each new sample you analyze, repeat
step 2 to C. 4
.
Output and Data Processing
USB Data Port
Model
1100
has a USB port which will allow you to interface with a Windows
®
based computer
for use with optional software, contact UNICO for details.
The
Spectrophotometer Application Software
is Windows-based software designed to operate
with your spectrophotometer using the USB port.
The software runs on a PC with Windows
®
XP/7/10 operating system installed. You can export data files collected from the
spectrophotometer into Excel
®
format if needed. The software offers two additional analytical
methods:
Standard Curve
and
Absorbance vs. Time Kinetics
. It
performs the following
methods for analysis:
•
Absorbance/%Transmittance/Concentration
Measure the Absorbance,
%Transmittance, Concentration/Standard, or Concentration/Factor at a single wavelength
within the range of
335
-
1000
nm.
•
Standard Curve
Create a calibration curve (choice of 4 curve fits) with up to 8 standard
solutions at a single wavelength to determine concentrations of unknown samples.
•
Absorbance vs. Time Kinetics
M
easure a sample’s absorbance change over a selected
period of time, store the results in a data table, and display the results graphically.
