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Chapter 6: Operation
Thermo Scientific Wellwash
®
4 Mk 2 User Manual Rev.2.3, Cat. no. 1507340
34
PAUSE
Set the pause between each wash cycle (Table 6.5). During the strip wash
mode, the pause is between each row. During the plate wash mode, the pause
is between each pass over the plate.
Note:
The times currently available are subject to approval and may be altered
to suit individual customer requirements.
Table 6.5 Pause (in seconds) between each washing cycle
Setting
0
1
2
3
4
5
6
7
Strip wash mode
(seconds)
0 1 2 3 4 5 6 7
Plate wash mode
(seconds)
0 5 10 15 20 25 30 40
Setting
8 9 A B C D E F
Strip wash mode
(seconds)
8 9 10 15 20 30 45 60
Plate wash mode
(seconds)
50 60 75 85 105 120 180 240
WASHES
Set the number of washes to be performed before a soak period (Table 6.6).
Table 6.6 Number of washes before soaking
Setting
0
1
2
3
4
5
6
7
Number of washes
0*)
1
2
3
4
5
6
7
Setting
8 9 A B C D E F
Number of washes
8
9
10
11
12
13
14
15
*) No wash, only aspirate
VOLUME
Set the volume dispensed during washing (Table 6.7).
Table 6.7 Washing volume (in µl)
Setting
0
1
2
3
4
5
6
7
Washing
volume
(µl) 0 50 100 150 200 250 300 350
Setting
8 9 A B C D E F
Washing
volume
(µl) 400 450 500 550 600 650 700 750
Note:
The dispense volume is less than the wash volume to avoid overfilling
the wells.
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