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Chapter 3: Introduction to the Wellwash 4 Mk 2
Thermo Scientific Wellwash
®
4 Mk 2 User Manual Rev.2.3, Cat. no. 1507340
9
3
Introduction to the Wellwash 4 Mk 2
The Wellwash 4 Mk 2 is a semiautomatic microplate washer, comprising pump
and washer units. The instrument is supplied with both 8- and 12-way wash
heads, four program cards and an accessory pack.
Your local Thermo Fisher Scientific representative can arrange instrument
training at the commissioning of the instrument for extra charge, if required.
3.1
Intended use
The Wellwash 4 Mk 2 is a microplate washer for 96-well plates. It can be used in
research or routine-test laboratories by trained personnel to wash Microtiter
microplates and strips in 96-well plate format that meet the SBS standards.
For verification of the entire system, it is recommended that Good Laboratory
Practices (GLP) be followed to guarantee reliable analyses.
Use for self-testing is excluded.
If the analyzing performance is critical to the medical diagnosis, the diagnostic
test result has to be ensured with internal quality contols or with an alternative
test.
3.2
Principle of operation
The instrument provides the following wash functions initiated through the
keypad and control switches or a preprogrammed wash card:
1.
Liquid dispensing into wells (microplate or microstrip)
2.
Liquid aspiration from wells (microplate or microstrip)
With their unique and proven design, the coaxial wash heads of the Wellwash
4 Mk 2 ensure excellent washing efficiency and low residual volumes of less
than 5
µ
l. For clean delivery of washing buffer, the inner dispensing tubes are
made of stainless steel. A continuous vacuum in the outer tube prevents cross
contamination and ensures complete liquid removal.
For fast and accurate selection of routine protocols, the Wellwash 4 Mk 2 comes
with four standard program cards (1 to 4 washing cycles) as well as a
programmable card. This switch card permits a quick selection of all wash
parameters, including soak times and final wash protocols.
The quick release bottle caps of the Wellwash 4 Mk 2 ensure easy buffer
changes. An additional rinse bottle can be connected, making it easier to purge
wash buffer from the wash head or change to a different wash buffer.
Automatic pump shutdown reduces wear and noise levels. If unused for 30
seconds, the wash pump of the Wellwash 4 Mk 2 automatically switches off.
3.3
Advantages of using Wellwash 4 Mk 2
The Wellwash 4 Mk 2 provides several advantages relating mainly to the
principle of operation in that it has:
•
Unique coaxial wash heads for trouble-free washing and low residual
volumes
•
8- and 12-way interchangeable wash heads
•
Easy to program
•
Excellent washing performance
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