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Chapter 6: Operation
Thermo Scientific Wellwash
®
4 Mk 2 User Manual Rev.2.3, Cat. no. 1507340
27
6.2
Washing a microplate
Ensure that the instrument is switched OFF.
Open the wash bottle cap (Fig. 6.1) very carefully to release any
residual pressure. Let the hissing sound end prior to opening the cap
all the way. Fill the wash bottle with appropriate washing reagent and
tighten the bottle cap firmly (see Fig. 5.12).
Select either one of the preprogrammed wash cards or the
programmable switch card (see Section 6.5).
Before inserting any program card, ensure that it is clean and dry.
Wiping the surfaces with a soft dry cloth will suffice. If any liquid is
present on the card, a malfunction of the program may occur.
Position the plate carrier on its slideway with the priming reservoir to
the rear under the wash head (see Fig. 5.13).
The POWER indicator on the keypad (Fig. 4.2) illuminates and a
countdown sequence will begin on the row indicator LEDs.
The PRIME indicator flashes until you prime the system (Section 6.3).
The priming function is complete when the PRIME indicator turns OFF.
If no further key entry is made within fifteen seconds of priming, then
the pump will automatically switch OFF. The instrument will then enter
the idle state, waiting for instructions from the keypad and program
card.
Check that the correct wash head (8-way or 12-way) is fitted.
Check that the plate is placed properly onto the plate carrier (Fig. 5.16).
Select the row number of the last row to be washed. If no row is
selected, the whole plate will be washed.
The instrument performs the wash sequence.
After pressing the START button, there may be a delay of up to 15
seconds before the run begins to allow the reagent and waste bottles
to reach the working pressure.
The plate carrier will advance into the washer aspirating and filling
selected rows in the sequence determined by the program card fitted.
On completion of the protocol, the plate carrier will come to rest with
the wash head lowered into the priming reservoir.
Fill the wash
bottle and
empty the
waste bottle
Turn the
instrument
ON
PRIME
Remove the
microplate
from the
plate carrier
Place the
microplate
onto the
plate carrier
START
Position the
plate carrier
for priming
Plug the
program
card into the
slot
1 –
12
Turn the
instrument
OFF
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